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VCAM1 / CD106 antibody

Anti-VCAM1 / CD106 antibody used in IHC (Paraffin sections) (IHC-P). GTX100562
Anti-VCAM1 / CD106 antibody used in Western Blot (WB). GTX100562
Anti-VCAM1 / CD106 antibody used in IHC (Paraffin sections) (IHC-P). GTX100562

Cat. No. GTX100562

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all VCAM1 / CD106 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Predict Reactivity

Rabbit, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human VCAM1 / CD106. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036476

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vascular cell adhesion molecule 1 , CD106 , INCAM-100

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VCAM1 / CD106 antibody used in IHC (Paraffin sections) (IHC-P). GTX100562

GTX100562 IHC-P Image

VCAM1 / CD106 antibody detects VCAM1 / CD106 protein at cell membrane in human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
VCAM1 / CD106 antibody (GTX100562) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VCAM1 / CD106 antibody used in Western Blot (WB). GTX100562

GTX100562 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCAM1 / CD106 antibody (GTX100562) diluted at 1:200000.

Anti-VCAM1 / CD106 antibody used in IHC (Paraffin sections) (IHC-P). GTX100562

GTX100562 IHC-P Image

VCAM1 / CD106 antibody detects VCAM1 / CD106 protein at cell membrane in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
VCAM1 / CD106 antibody (GTX100562) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169