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VMAT2 antibody [9E11]

Overexpression
Anti-VMAT2 antibody [9E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83635
Anti-VMAT2 antibody [9E11] used in Western Blot (WB). GTX83635
Anti-VMAT2 antibody [9E11] used in IHC (Paraffin sections) (IHC-P). GTX83635
Anti-VMAT2 antibody [9E11] used in Flow cytometry (FACS). GTX83635
Anti-VMAT2 antibody [9E11] used in Western Blot (WB). GTX83635

Cat. No. GTX83635

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 9E11

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse
Package
100 μl ($539)
See all VMAT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human SLC18A2 expression vector .

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 18 member A2 , PKDYS2 , SVAT , SVMT , VAT2 , VMAT2

Background

Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis.

Database

Research Area

DATA IMAGES

Anti-VMAT2 antibody [9E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83635

GTX83635 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with VMAT2 plasmid using GTX83635 VMAT2 antibody [9E11].

Anti-VMAT2 antibody [9E11] used in Western Blot (WB). GTX83635

GTX83635 WB Image

Overexpression

WB analysis of HEK293T cells transfected with VMAT2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83635 VMAT2 antibody [9E11].
Loading : 5 ug per lane

Anti-VMAT2 antibody [9E11] used in IHC (Paraffin sections) (IHC-P). GTX83635

GTX83635 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX83635 VMAT2 antibody [9E11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-VMAT2 antibody [9E11] used in Flow cytometry (FACS). GTX83635

GTX83635 FACS Image

FACS analysis of HEK293T cells transfected with either VMAT2 plasmid(Red) or empty vector control plasmid(Blue) using GTX83635 VMAT2 antibody [9E11].

Anti-VMAT2 antibody [9E11] used in Western Blot (WB). GTX83635

GTX83635 WB Image

WB analysis of various samples using GTX83635 VMAT2 antibody [9E11].
Loading : 10 ug per lane
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 539