APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-3μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX26051-PEP).
Product Note
This antibody is expected to recognize isoform 1 (NP_004887.2) only.
Predict Reactivity
Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-SPESQASAEQPEM, from the C Terminus of the protein sequence according to NP_004887.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
VPS26, retromer complex component A , HB58 , Hbeta58 , PEP8A , VPS26
Cellular Localization
Cytoplasm , Endosome membrane
Background
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX26051 WB Image
WB analysis of human kidney lysate using GTX26051 VPS26A antibody, C-term. Dilution : 1μg/ml Loading : 35μg protein in RIPA buffer
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GTX26051 WB Image
WB analysis of HEK293 overexpressing VPS26A (mock transfection in first lane) using GTX26051 VPS26A antibody, C-term. Loading : 35μg protein in RIPA buffer
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REFERENCE
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REVIEW
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