APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.03-0.1μg/ml |
| 2.5μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89447-PEP).
Predict Reactivity
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-FQKKKPKEQQKLES, from the internal region of the protein sequence according to NP_009190.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
vacuolar protein sorting 45 homolog , H1 , H1VPS45 , SCN5 , VPS45A , VPS45B , VPS54A , VSP45 , VSP45A
Cellular Localization
Golgi apparatus membrane , Endosome membrane
Background
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec1 domain family, and shows a high degree of sequence similarity to mouse, rat and yeast Vps45. The exact function of this gene is not known, but its high expression in peripheral blood mononuclear cells suggests a role in trafficking proteins, including inflammatory mediators. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2013]
Database
Research Area
DATA IMAGES
|
GTX89447 WB Image
WB analysis of human placenta lysate using GTX89447 VPS45 antibody, Internal.
Dilution : 0.03μg/ml
Loading : 35μg protein in RIPA buffer
|
|
|
GTX89447 IHC-P Image
IHC-P analysis of human spleen using GTX89447 VPS45 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
|
REFERENCE
There are currently no references for VPS45 antibody, Internal (GTX89447). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for VPS45 antibody, Internal (GTX89447). Be the first to share your experience with this product.
