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WHSC2 antibody [6B11H8]

Anti-WHSC2 antibody [6B11H8] used in IHC (Paraffin sections) (IHC-P). GTX60733
Anti-WHSC2 antibody [6B11H8] used in ELISA (ELISA). GTX60733
Anti-WHSC2 antibody [6B11H8] used in Western Blot (WB). GTX60733
Anti-WHSC2 antibody [6B11H8] used in IHC (Paraffin sections) (IHC-P). GTX60733
Anti-WHSC2 antibody [6B11H8] used in Flow cytometry (FACS). GTX60733
Anti-WHSC2 antibody [6B11H8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60733
Anti-WHSC2 antibody [6B11H8] used in Western Blot (WB). GTX60733

Cat. No. GTX60733

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6B11H8

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Rat
Package
100 μg ($409)
See all WHSC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human WHSC2 (AA: 280-511) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

negative elongation factor complex member A , NELF-A , P/OKcl.15 , WHSC2

Cellular Localization

Nucleus

Background

This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES

Anti-WHSC2 antibody [6B11H8] used in IHC (Paraffin sections) (IHC-P). GTX60733

GTX60733 IHC-P Image

IHC-P analysis of colon cancer tissue using GTX60733 WHSC2 antibody [6B11H8].

Anti-WHSC2 antibody [6B11H8] used in ELISA (ELISA). GTX60733

GTX60733 ELISA Image

ELISA analysis of antigen using GTX60733 WHSC2 antibody [6B11H8].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-WHSC2 antibody [6B11H8] used in Western Blot (WB). GTX60733

GTX60733 WB Image

WB analysis of Jurkat (1), HeLa (2), HEK293 (3), A549 (5), SPC-A-1 (6) cell lysate, and Rat brain (4) tissue lysate using GTX60733 WHSC2 antibody [6B11H8].

Anti-WHSC2 antibody [6B11H8] used in IHC (Paraffin sections) (IHC-P). GTX60733

GTX60733 IHC-P Image

IHC-P analysis of ovarian cancer tissue using GTX60733 WHSC2 antibody [6B11H8].

Anti-WHSC2 antibody [6B11H8] used in Flow cytometry (FACS). GTX60733

GTX60733 FACS Image

FACS analysis of HEK293 cells using GTX60733 WHSC2 antibody [6B11H8].
Green : WHSC2
Purple : negative control

Anti-WHSC2 antibody [6B11H8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60733

GTX60733 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60733 WHSC2 antibody [6B11H8].
Green : WHSC2
Blue: DRAQ5 fluorescent DNA dye

Anti-WHSC2 antibody [6B11H8] used in Western Blot (WB). GTX60733

GTX60733 WB Image

WB analysis of HEK293 (1) and WHSC2 (AA: 280-511)-hIgGFc transfected HEK293 (2) cell lysate using GTX60733 WHSC2 antibody [6B11H8].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409