GeneTex
United States (US)

WNT4 antibody

Overexpression
GTX101085_39995_20150206_WB_19011506_693.jpg
GTX101085_39995_IHC_M_19011506_958.jpg
GTX101085_39995_20150206_WB_B_19011506_884.jpg
GTX101085_39995_WB_B_19011506_151.jpg
GTX101085_39995_IHC_19011506_852.jpg

Cat No. GTX101085

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

293T , HepG2 , WNT4 transfected 293T lysate , V5-human Wnt4-transfected 293T

Predict Reactivity

Chicken, Japanese Medaka, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WNT4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Wnt Family Member 4,Serkal,Wnt-4,Wnt4

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101085_39995_20150206_WB_19011506_693.jpg

GTX101085 WB Image

WNT4 antibody detects WNT4 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with WNT4 antibody (GTX101085) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101085_39995_IHC_M_19011506_958.jpg

GTX101085 IHC-P Image

WNT4 antibody detects WNT4 protein at secreted on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
WNT4 antibody (GTX101085) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101085_39995_20150206_WB_B_19011506_884.jpg

GTX101085 WB Image

Overexpression

WNT4 antibody detects WNT4 protein by western blot analysis. Non-transfected (-) and Wnt4-transfected (+, including V5-tag) 293T whole cell extracts (100 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with WNT4 antibody (GTX101085) at a dilution of 1:2500.
The V5 was used as internal control (GTX117997, 1:2500) shown at the bottom panel.
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101085_39995_WB_B_19011506_151.jpg

GTX101085 WB Image

Overexpression

Sample (30 µg of whole cell lysate)
A: Non-transfected 293T lysates
B: WNT4 transfected 293T lysates
10% SDS PAGE
GTX101085 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101085_39995_IHC_19011506_852.jpg

GTX101085 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using WNT4(GTX101085) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf