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Wnt11 antibody

Overexpression
Anti-Wnt11 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105971
Anti-Wnt11 antibody used in Western Blot (WB). GTX105971
Anti-Wnt11 antibody used in IHC (Paraffin sections) (IHC-P). GTX105971
Anti-Wnt11 antibody used in Western Blot (WB). GTX105971
Anti-Wnt11 antibody used in Western Blot (WB). GTX105971
Anti-Wnt11 antibody used in IHC (Paraffin sections) (IHC-P). GTX105971
Anti-Wnt11 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105971
Anti-Wnt11 antibody used in Western Blot (WB). GTX105971
Anti-Wnt11 antibody used in Western Blot (WB). GTX105971
Anti-Wnt11 antibody used in Western Blot (WB). GTX105971
Anti-Wnt11 antibody used in Western Blot (WB). GTX105971

Cat. No. GTX105971

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Wnt11 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Predict Reactivity

Rabbit, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WNT11. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952580

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Wnt family member 11 , HWNT11

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 97%, 85%, and 63% amino acid identity with mouse, chicken, and Xenopus Wnt11 protein, respectively. This gene may play roles in the development of skeleton, kidney and lung, and is considered to be a plausible candidate gene for High Bone Mass Syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Wnt11 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105971

GTX105971 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using WNT11(GTX105971) antibody at 1:200 dilution.

Anti-Wnt11 antibody used in Western Blot (WB). GTX105971

GTX105971 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody (GTX105971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Wnt11 antibody used in IHC (Paraffin sections) (IHC-P). GTX105971

GTX105971 IHC-P Image

WNT11 antibody detects WNT11 protein at secreted on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
WNT11 antibody (GTX105971) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Wnt11 antibody used in Western Blot (WB). GTX105971

GTX105971 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody (GTX105971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Wnt11 antibody used in Western Blot (WB). GTX105971

GTX105971 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody (GTX105971) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Wnt11 antibody used in IHC (Paraffin sections) (IHC-P). GTX105971

GTX105971 IHC-P Image

Immunohistochemical analysis of paraffin-embedded lung SCC xenograft, using WNT11(GTX105971) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Wnt11 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105971

GTX105971 ICC/IF Image

WNT11 antibody detects WNT11 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: WNT11 protein stained by WNT11 antibody (GTX105971) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Wnt11 antibody used in Western Blot (WB). GTX105971

GTX105971 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody (GTX105971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Wnt11 antibody used in Western Blot (WB). GTX105971

GTX105971 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody (GTX105971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Wnt11 antibody used in Western Blot (WB). GTX105971

GTX105971 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody (GTX105971) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Wnt11 antibody used in Western Blot (WB). GTX105971

GTX105971 WB Image

Overexpression

The data was published in the journal EMBO J in 2018.PMID: 29378775

REFERENCE

Application Reference

Mortoglou M et al. Int J Mol Sci 2022; 23 (3) microRNA-21 Regulates Stemness in Pancreatic Ductal Adenocarcinoma Cells.
Application : ICC/IF
Reactivity : Human
Yan Zou et al. Cell Death Dis 2021; 12 (10) : 877 Cardiac Wnt5a and Wnt11 promote fibrosis by the crosstalk of FZD5 and EGFR signaling under pressure overload.
Application : WB
Reactivity : Human, Mouse
Arisan ED et al. Int J Mol Sci 2021; 22 (4) MiR-21 Is Required for the Epithelial-Mesenchymal Transition in MDA-MB-231 Breast Cancer Cells.
Application : WB
Reactivity : Human
Gorro?o-Etxebarria I et al. Cancers (Basel) 2019; 11 (7) Wnt-11 as a Potential Prognostic Biomarker and Therapeutic Target in Colorectal Cancer.
Application : IHC-P
Reactivity : Human
Glaeser K et al. EMBO J 2018; 37 (4) ERAD-dependent control of the Wnt secretory factor Evi.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169