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Wnt16 antibody

Overexpression
Anti-Wnt16 antibody used in Western Blot (WB). GTX128468
Anti-Wnt16 antibody used in Western Blot (WB). GTX128468
Anti-Wnt16 antibody used in Western Blot (WB). GTX128468

Cat. No. GTX128468

Reference 3
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Wnt16 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WNT16. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885783

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Wnt family member 16

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It contains two transcript variants diverging at the 5' termini. These two variants are proposed to be the products of separate promoters and not to be splice variants from a single promoter. They are differentially expressed in normal tissues, one of which (variant 2) is expressed at significant levels only in the pancreas, whereas another one (variant 1) is expressed more ubiquitously with highest levels in adult kidney, placenta, brain, heart, and spleen. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Wnt16 antibody used in Western Blot (WB). GTX128468

GTX128468 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt16 antibody (GTX128468) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Wnt16 antibody used in Western Blot (WB). GTX128468

GTX128468 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with WNT16 antibody (GTX128468) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Wnt16 antibody used in Western Blot (WB). GTX128468

GTX128468 WB Image

Overexpression

The data was published in the journal EMBO J in 2018.PMID: 29378775

REFERENCE

Application Reference

Karvonen H et al. Oncogene 2019; 38 (17) : 3288-3300 Wnt5a and ROR1 activate non-canonical Wnt signaling via RhoA in TCF3-PBX1 acute lymphoblastic leukemia and highlight new treatment strategies via Bcl-2 co-targeting.
Application : WB
Reactivity : Human
Glaeser K et al. EMBO J 2018; 37 (4) ERAD-dependent control of the Wnt secretory factor Evi.
Application : WB
Reactivity : Human
Aso E et al. J Alzheimers Dis 2016; Delineating the Efficacy of a Cannabis-Based Medicine at Advanced Stages of Dementia in a Murine Model.
Application : WB
Submit a Reference

REVIEW

Wnt16 antibody Cat. No. GTX128468

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Wnt16 antibody used in Western Blot (WB). GTX128468
Date : Anonymous submitted on 19-Aug-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : human Wnt16 transfected 293T cells
Amount used : 30μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, 14Hr
Lane Description : Lane1: 293T cells transfected with empty vector, Lane2: 293T cells transfected with human WNT16
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169