GeneTex

  • Country / Location Selection

United States (US)

Wnt4 antibody, Internal

Anti-Wnt4 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81775
Anti-Wnt4 antibody, Internal used in Western Blot (WB). GTX81775
Anti-Wnt4 antibody, Internal used in Flow cytometry (FACS). GTX81775
Anti-Wnt4 antibody, Internal used in Western Blot (WB). GTX81775

Cat. No. GTX81775

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
200 μl ($399)
See all Wnt4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
IHC-P 1:10-1:50
FACS 1:25
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 211-239 amino acids from the Central region of human WNT4.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Wnt family member 4 , SERKAL , WNT-4

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Wnt4 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81775

GTX81775 IHC-P Image

IHC-P analysis of human bladder carcinoma using GTX81775 WNT4 antibody, Internal.

Anti-Wnt4 antibody, Internal used in Western Blot (WB). GTX81775

GTX81775 WB Image

WB analysis of various samples using GTX81775 WNT4 antibody, Internal.
Lane 1: HeLa whole cell lysate
Lane 2: human testis lysate
Lane 3: mouse bladder lysate
Lane 4: rat lung lysate
Loading : 20 μg per lane
Dilution : 1:2000

Anti-Wnt4 antibody, Internal used in Flow cytometry (FACS). GTX81775

GTX81775 FACS Image

FACS analysis of MCF-7 cells using GTX81775 WNT4 antibody, Internal.
Green : WNT4
Blue : Isotype control
Fixation : 2% paraformaldehyde (PFA) (10 min)
Permeabilization : 90% methanol (10 min)
Dilution : 1:25

Anti-Wnt4 antibody, Internal used in Western Blot (WB). GTX81775

GTX81775 WB Image

WB analysis of various samples using GTX81775 WNT4 antibody, Internal.
Lane 1: MDA-MB-231 whole cell lysate
Lane 2: mouse bladder lysate
Loading : 20 μg per lane
Dilution : 1:2000

REFERENCE

There are currently no references for Wnt4 antibody, Internal (GTX81775). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Wnt4 antibody, Internal (GTX81775). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399