Wnt6 antibody [HL1568]

Cat. No. GTX637043

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1568
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Rat
Package	100 μl ($399), 25 μl ($169)

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Datasheet

View product citations for antibody GTX637043 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	Assay dependent
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Product Note

This antibody is specific for human Wnt6 protein, and it does not cross react with human Wnt1, Wnt2, Wnt2b, Wnt3, Wnt3a, Wnt4, Wnt5a, Wnt7a, Wnt7b, Wnt8a, Wnt8b, Wnt9a, Wnt9b, Wnt10a, Wnt10b, and Wnt16 protein.

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Wnt6. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Wnt family member 6

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is overexpressed in cervical cancer cell line and strongly coexpressed with another family member, WNT10A, in colorectal cancer cell line. The gene overexpression may play key roles in carcinogenesis. This gene and the WNT10A gene are clustered in the chromosome 2q35 region. The protein encoded by this gene is 97% identical to the mouse Wnt6 protein at the amino acid level. [provided by RefSeq, Jul 2008]

Database

Gene ID: 7475 WNT6
UniProt: Q9Y6F9 WNT6

Research Area

DATA IMAGES

GTX637043 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt6 antibody [HL1568] (GTX637043) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX637043 IHC-P Image

Wnt6 antibody [HL1568] detects Wnt6 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat adrenal gland.
Wnt6 stained by Wnt6 antibody [HL1568] (GTX637043) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX637043 ICC/IF Image

Wnt6 antibody [HL1568] detects Wnt6 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Wnt6 stained by Wnt6 antibody [HL1568] (GTX637043) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations