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XPA antibody [5F12]

Orthogonal
Anti-XPA antibody [5F12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00704
Anti-XPA antibody [5F12] used in Western Blot (WB). GTX00704
Anti-XPA antibody [5F12] used in Western Blot (WB). GTX00704

Cat. No. GTX00704

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5F12

Isotype

 IgG2b

Application

 WB, ICC/IF, ELISA, Neutralizing/Inhibition

Reactivity

 Human
Package
100 μg ($399)
See all XPA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
ELISA Assay dependent
Neutralizing/Inhibition Assay dependent

Note :


It shows the inhibition of in vitro excision repair reaction and inhibition of XPA interaction with ERCC1 and TFIIH.
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

HeLa cells

Negative Control

XP12ROSV cells

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human XPA protein.
Epiopte: 30-47 amino acids

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

XPA, DNA damage recognition and repair factor , XP1 , XPAC

Cellular Localization

Nucleus

Background

This gene encodes a zinc finger protein plays a central role in nucleotide excision repair (NER), a specialized type of DNA repair. NER is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens and chemotherapeutic drugs. The encoded protein interacts with DNA and several NER proteins, acting as a scaffold to assemble the NER incision complex at sites of DNA damage. Mutations in this gene cause Xeroderma pigmentosum complementation group A (XP-A), an autosomal recessive skin disorder featuring hypersensitivity to sunlight and increased risk for skin cancer. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-XPA antibody [5F12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00704

GTX00704 ICC/IF Image

ICC/IF analysis of GM0637 cells irradiated with UV at 20 J/m2 using GTX00704 XPA antibody [5F12].
Dilution : 1:100

Anti-XPA antibody [5F12] used in Western Blot (WB). GTX00704

GTX00704 WB Image

WB analysis of HeLa whole cell lysate using GTX00704 XPA antibody [5F12].
Dilution : 1:2000

Anti-XPA antibody [5F12] used in Western Blot (WB). GTX00704

GTX00704 WB Image

Orthogonal

WB analysis of various samples using GTX00704 XPA antibody [5F12].
Lane 1 : HeLa whole cell lysate (XpA wild-type)
Lane 2 : XP12ROSV whole cell lysate (XpA deficient)
Dilution : 1:2000

REFERENCE

Clone Reference

Tanaka K et al. Nature. 1990; 348 (6296) Analysis of a human DNA excision repair gene involved in group A xeroderma pigmentosum and containing a zinc-finger domain.
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399