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YAP1 antibody

KO/KD Overexpression
Anti-YAP1 antibody used in Western Blot (WB). GTX130444
Anti-YAP1 antibody used in Western Blot (WB). GTX130444
Anti-YAP1 antibody used in Western Blot (WB). GTX130444
Anti-YAP1 antibody used in Immunoprecipitation (IP). GTX130444
Anti-YAP1 antibody used in Western Blot (WB). GTX130444
Anti-YAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130444

Cat. No. GTX130444

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all YAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

A431 , HepG2 , zebrafish brain , zebrafish eye , HeLa , YAP1-transfected 293T

Predict Reactivity

Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human YAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886270

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Yes associated protein 1 , COB1 , YAP , YAP2 , YAP65 , YKI

Background

This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-YAP1 antibody used in Western Blot (WB). GTX130444

GTX130444 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 antibody (GTX130444) diluted at 1:500.

Anti-YAP1 antibody used in Western Blot (WB). GTX130444

GTX130444 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 antibody (GTX130444) diluted at 1:1000.

Anti-YAP1 antibody used in Western Blot (WB). GTX130444

GTX130444 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 antibody (GTX130444) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-YAP1 antibody used in Immunoprecipitation (IP). GTX130444

GTX130444 IP Image

Immunoprecipitation of YAP1 protein from HeLa whole cell extracts using 5 μg of YAP1 antibody (GTX130444).
Western blot analysis was performed using YAP1 antibody (GTX130444).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-YAP1 antibody used in Western Blot (WB). GTX130444

GTX130444 WB Image

YAP1 antibody detects YAP1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 antibody (GTX130444) diluted by 1:1000.

Anti-YAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130444

GTX130444 IHC-P Image

YAP1 antibody detects YAP1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human glioblastoma.
YAP1 stained by YAP1 antibody (GTX130444) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169