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YTHDF2 antibody [HL1354]

Recombinant antibody Overexpression
Anti-YTHDF2 antibody [HL1354] used in IHC (Paraffin sections) (IHC-P). GTX636774
Anti-YTHDF2 antibody [HL1354] used in IHC (Paraffin sections) (IHC-P). GTX636774
Anti-YTHDF2 antibody [HL1354] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636774
Anti-YTHDF2 antibody [HL1354] used in Western Blot (WB). GTX636774
Anti-YTHDF2 antibody [HL1354] used in Western Blot (WB). GTX636774
Anti-YTHDF2 antibody [HL1354] used in Western Blot (WB). GTX636774

Cat. No. GTX636774

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1354

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all YTHDF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Product Note

This antibody is specific for human YTHDF2 protein, and it does not cross react with human YTHDF1 protein.

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human YTHDF2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

YTH N6-methyladenosine RNA binding protein 2 , CAHL , HGRG8 , NY-REN-2

Cellular Localization

Cytoplasm, cytosol,Nucleus

Background

This gene encodes a member of the YTH (YT521-B homology) superfamily containing YTH domain. The YTH domain is typical for the eukaryotes and is particularly abundant in plants. The YTH domain is usually located in the middle of the protein sequence and may function in binding to RNA. In addition to a YTH domain, this protein has a proline rich region which may be involved in signal transduction. An Alu-rich domain has been identified in one of the introns of this gene, which is thought to be associated with human longevity. In addition, reciprocal translocations between this gene and the Runx1 (AML1) gene on chromosome 21 has been observed in patients with acute myeloid leukemia. This gene was initially mapped to chromosome 14, which was later turned out to be a pseudogene. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Oct 2012]

Database

DATA IMAGES

Anti-YTHDF2 antibody [HL1354] used in IHC (Paraffin sections) (IHC-P). GTX636774

GTX636774 IHC-P Image

YTHDF2 antibody [HL1354] detects YTHDF2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
YTHDF2 stained by YTHDF2 antibody [HL1354] (GTX636774) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-YTHDF2 antibody [HL1354] used in IHC (Paraffin sections) (IHC-P). GTX636774

GTX636774 IHC-P Image

YTHDF2 antibody [HL1354] detects YTHDF2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
YTHDF2 stained by YTHDF2 antibody [HL1354] (GTX636774) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-YTHDF2 antibody [HL1354] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636774

GTX636774 ICC/IF Image

YTHDF2 antibody [HL1354] detects YTHDF2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: YTHDF2 stained by YTHDF2 antibody [HL1354] (GTX636774) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-YTHDF2 antibody [HL1354] used in Western Blot (WB). GTX636774

GTX636774 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with YTHDF2 antibody [HL1354] (GTX636774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-YTHDF2 antibody [HL1354] used in Western Blot (WB). GTX636774

GTX636774 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with YTHDF2 antibody [HL1354] (GTX636774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-YTHDF2 antibody [HL1354] used in Western Blot (WB). GTX636774

GTX636774 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with YTHDF2 antibody [HL1354] (GTX636774) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations