ZAK antibody [N1N2], N-term

Cat. No. GTX111477

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all ZAK products

Datasheet
Document

View product citations for antibody GTX111477 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

91 kDa. ( Note )

Positive Control

Molt-4

Predict Reactivity

Mouse, Zebrafish, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ZAK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952639

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase kinase 20 , AZK , CNM6 , MLK7 , MLT , MLTK , MLTKalpha , MLTKbeta , MRK , SFMMP , ZAK , mlklak , pk

Cellular Localization

Cytoplasm , Nucleus

Background

This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Gene ID: 51776 MAP3K20
UniProt: Q9NYL2 MAP3K20

Research Area

DATA IMAGES

GTX111477 IHC-P Image

ZAK antibody [N1N2], N-term detects ZAK protein at on Mahlarvu xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Mahlarvu xenograft.
ZAK antibody [N1N2], N-term (GTX111477) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX111477 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX111477 diluted at 1:1000

GTX111477 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using ZAK(GTX111477) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty