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ZC3HAV1 antibody [N3C2], Internal

Anti-ZC3HAV1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX120134
Anti-ZC3HAV1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX120134
Anti-ZC3HAV1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120134
Anti-ZC3HAV1 antibody [N3C2], Internal used in Western Blot (WB). GTX120134
Anti-ZC3HAV1 antibody [N3C2], Internal used in Western Blot and Immunoprecipitation (WB IP). GTX120134

Cat. No. GTX120134

Reference 9

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ZC3HAV1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

101 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZC3HAV1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721153

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger CCCH-type containing, antiviral 1 , ARTD13 , FLB6421 , PARP13 , ZAP , ZC3H2 , ZC3HDC2

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a CCCH-type zinc finger protein that is thought to prevent infection by retroviruses. Studies of the rat homolog indicate that the protein may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection. Alternative splicing occurs at this locus and two variants, each encoding distinct isoforms, are described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ZC3HAV1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX120134

GTX120134 IHC-P Image

ZC3HAV1 antibody [N3C2], Internal detects ZC3HAV1 protein at cytoplasm in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
ZC3HAV1 antibody [N3C2], Internal (GTX120134) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZC3HAV1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX120134

GTX120134 IHC-P Image

ZC3HAV1 antibody [N3C2], Internal detects ZC3HAV1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
ZC3HAV1 stained by ZC3HAV1 antibody [N3C2], Internal (GTX120134) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZC3HAV1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120134

GTX120134 ICC/IF Image

ZC3HAV1 antibody [N3C2], Internal detects ZC3HAV1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZC3HAV1 stained by ZC3HAV1 antibody [N3C2], Internal (GTX120134) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-ZC3HAV1 antibody [N3C2], Internal used in Western Blot (WB). GTX120134

GTX120134 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3HAV1 antibody [N3C2], Internal (GTX120134) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZC3HAV1 antibody [N3C2], Internal used in Western Blot and Immunoprecipitation (WB IP). GTX120134

GTX120134 WB IP Image

The data was published in the 2018 in PLoS Pathog. PMID: 30016363

REFERENCE

Application Reference

Popovi? B et al. Cell Rep 2023; 42 (5) : 112419 Time-dependent regulation of cytokine production by RNA binding proteins defines T cell effector function.
Okudera M et al. Neuroimmunomodulation 2021; : 44571 Expression of Zinc-Finger Antiviral Protein in hCMEC/D3 Human Cerebral Microvascular Endothelial Cells: Effect of a Toll-Like Receptor 3 Agonist.
Kmiec D et al. PLoS Pathog 2021; 17 (10) : e1009726 S-farnesylation is essential for antiviral activity of the long ZAP isoform against RNA viruses with diverse replication strategies.
Application : WB
Reactivity : Human
Rodriguez KM et al. Elife 2021; 10 Chemical genetics and proteome-wide site mapping reveal cysteine MARylation by PARP-7 on immune-relevant protein targets.
Application : WB
Nchioua R et al. mBio 2020; 11 (5) SARS-CoV-2 Is Restricted by Zinc Finger Antiviral Protein despite Preadaptation to the Low-CpG Environment in Humans.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Popovi? B et al. Cell Rep 2023; 42 (5):112419 Time-dependent regulation of cytokine production by RNA binding proteins defines T cell effector function.
Okudera M et al. Neuroimmunomodulation 2021;:44571 Expression of Zinc-Finger Antiviral Protein in hCMEC/D3 Human Cerebral Microvascular Endothelial Cells: Effect of a Toll-Like Receptor 3 Agonist.
Kmiec D et al. PLoS Pathog 2021; 17 (10):e1009726 S-farnesylation is essential for antiviral activity of the long ZAP isoform against RNA viruses with diverse replication strategies.
Application : WB
Reactivity : Human
Rodriguez KM et al. Elife 2021; 10 Chemical genetics and proteome-wide site mapping reveal cysteine MARylation by PARP-7 on immune-relevant protein targets.
Application : WB
Nchioua R et al. mBio 2020; 11 (5) SARS-CoV-2 Is Restricted by Zinc Finger Antiviral Protein despite Preadaptation to the Low-CpG Environment in Humans.
Application : WB
Reactivity : Human
Kmiec D et al. mBio 2020; 11 (1) CpG Frequency in the 5' Third of the env Gene Determines Sensitivity of Primary HIV-1 Strains to the Zinc-Finger Antiviral Protein.
Application : WB
Reactivity : Human
Chiu HP et al. PLoS Pathog 2018; 14 (7):e1007166 Inhibition of Japanese encephalitis virus infection by the host zinc-finger antiviral protein.
Application : WB
Burke JM et al. Nucleic Acids Res 2017; 45 (17):e154 Expression of short hairpin RNAs using the compact architecture of retroviral microRNA genes.
Burke J.M. et al. bioRxiv 2017; (Epub) Expression of short hairpin RNAs using the compact architecture of retroviral microRNA genes.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169