GeneTex

  • Country / Location Selection

United States (US)

ZEB1 antibody

KO/KD Orthogonal
Anti-ZEB1 antibody used in Western Blot (WB). GTX55847
Anti-ZEB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55847
Anti-ZEB1 antibody used in Western Blot (WB). GTX55847
Anti-ZEB1 antibody used in Western Blot (WB). GTX55847

Cat. No. GTX55847

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all ZEB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein of human ZEB1

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887939

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger E-box binding homeobox 1 , AREB6 , BZP , DELTAEF1 , FECD6 , NIL2A , PPCD3 , TCF8 , ZFHEP , ZFHX1A

Cellular Localization

Nucleus

Background

This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-ZEB1 antibody used in Western Blot (WB). GTX55847

GTX55847 WB Image

WB analysis of Jurkat cell lysate using GTX55847 ZEB1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-ZEB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55847

GTX55847 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX55847 ZEB1 antibody.
Dilution : 1:200

Anti-ZEB1 antibody used in Western Blot (WB). GTX55847

GTX55847 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX55847 ZEB1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-ZEB1 antibody used in Western Blot (WB). GTX55847

GTX55847 WB Image

Orthogonal

The data was published in the journal J Exp Clin Cancer Res in 2019. PMID: 31661013

REFERENCE

Application Reference

Yu-Ting Yen et al. Int J Mol Sci 2021; 23 (1) Down-Regulation of miR-194-5p for Predicting Metastasis in Breast Cancer Cells.
Application : WB
Reactivity : Human
Kowolik CM et al. J Exp Clin Cancer Res 2019; 38 (1) : 431 Attenuation of hedgehog/GLI signaling by NT1721 extends survival in pancreatic cancer.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for ZEB1 antibody (GTX55847). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399