GeneTex
United States (US)

ZO-1 antibody - VetSignal

Anti-ZO-1 antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135090

Cat No. GTX135090

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application ICC/IF
Reactivity Dog
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

198 kDa. ( Note )

Predict Reactivity

Human, Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to dog ZO-1.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tight Junction Protein 1,Zo-1,Zo1,Tjp1

Cellular Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell junction , tight junction

Background

The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.
DATA IMAGES
Anti-ZO-1 antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135090

GTX135090 ICC/IF Image

ZO-1 antibody detects ZO-1 protein at cell membrane by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZO-1 stained by ZO-1 antibody (GTX135090) diluted at 1:1000.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319