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alpha 1a Adrenergic Receptor antibody

Anti-alpha 1a Adrenergic Receptor antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100190
Anti-alpha 1a Adrenergic Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX100190
Anti-alpha 1a Adrenergic Receptor antibody used in Western Blot (WB). GTX100190
Anti-alpha 1a Adrenergic Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX100190
Anti-alpha 1a Adrenergic Receptor antibody used in Western Blot (WB). GTX100190
Anti-alpha 1a Adrenergic Receptor antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100190
Anti-alpha 1a Adrenergic Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX100190

Cat. No. GTX100190

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Cat
Package
100 μl ($399),
25 μl ($169)
See all alpha 1a Adrenergic Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

Raji , Jurkat , Raji , NCI-H929

Predict Reactivity

Rabbit, Bovine, Dog, Guinea pig, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human alpha 1a Adrenergic Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1080925

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adrenoceptor alpha 1A , ADRA1C , ADRA1L1 , ALPHA1AAR

Cellular Localization

Cell membrane

Background

Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-alpha 1a Adrenergic Receptor antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100190

GTX100190 ICC/IF Image

alpha 1a Adrenergic Receptor antibody detects alpha 1a Adrenergic Receptor protein at cytoplasm by immunofluorescent analysis.
Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha 1a Adrenergic Receptor stained by alpha 1a Adrenergic Receptor antibody (GTX100190) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-alpha 1a Adrenergic Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX100190

GTX100190 IHC-P Image

alpha 1a Adrenergic Receptor antibody detects alpha 1a Adrenergic Receptor protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
alpha 1a Adrenergic Receptor antibody (GTX100190) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha 1a Adrenergic Receptor antibody used in Western Blot (WB). GTX100190

GTX100190 WB Image

Sample(30 μg of whole cell lysate)
A:Raji (GTX27908)
10% SDS PAGE
GTX100190 diluted at 1:1000

Anti-alpha 1a Adrenergic Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX100190

GTX100190 IHC-P Image

alpha 1a Adrenergic Receptor antibody detects alpha 1a Adrenergic Receptor protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
alpha 1a Adrenergic Receptor antibody (GTX100190) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha 1a Adrenergic Receptor antibody used in Western Blot (WB). GTX100190

GTX100190 WB Image

alpha 1a Adrenergic Receptor antibody detects alpha 1a Adrenergic Receptor protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
alpha 1a Adrenergic Receptor antibody (GTX100190) dilution: 1:1000

Anti-alpha 1a Adrenergic Receptor antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100190

GTX100190 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep3B, using alpha 1a Adrenergic Receptor(GTX100190) antibody at 1:500 dilution.

Anti-alpha 1a Adrenergic Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX100190

GTX100190 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human heart, using alpha 1a Adrenergic Receptor(GTX100190) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Choi BR et al. Drug Des Devel Ther 2018; 12 : 1855-1863 Additive effect of oral LDD175 to * and * in a benign prostate hyperplasia rat model.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169