APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:2000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
The clone 2E8 is specific for crystallin alpha B, but does not cross crystallin alpha A.
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone 2E8 is derived from hybridization of mouse SP4/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant Human Crystallin alpha B.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
crystallin alpha B , CMD1II , CRYA2 , CRYAB , CTPP2 , CTRCT16 , HELS101 , HSPB5 , MFM2 , alpha B Crystallin
Cellular Localization
Cytoplasm,Nucleus
Background
Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2019]
Database
Research Area
DATA IMAGES
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GTX57544 WB Image
WB analysis of various samples using GTX57544 alpha B Crystallin antibody. Samples : Recombinant Human crystallin alpha A (alphaA) and Mouse eye tissue lysate (ME) Dilution : 1:1000
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GTX57544 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX57544 alpha B Crystallin antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100
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REFERENCE
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REVIEW
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