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alpha B Crystallin antibody [6D11]

Overexpression
Anti-alpha B Crystallin antibody [6D11] used in IHC (Paraffin sections) (IHC-P). GTX84657
Anti-alpha B Crystallin antibody [6D11] used in Flow cytometry (FACS). GTX84657
Anti-alpha B Crystallin antibody [6D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84657
Anti-alpha B Crystallin antibody [6D11] used in IHC (Paraffin sections) (IHC-P). GTX84657
Anti-alpha B Crystallin antibody [6D11] used in Western Blot (WB). GTX84657
Anti-alpha B Crystallin antibody [6D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84657
Anti-alpha B Crystallin antibody [6D11] used in Western Blot (WB). GTX84657
Anti-alpha B Crystallin antibody [6D11] used in Western Blot (WB). GTX84657
Anti-alpha B Crystallin antibody [6D11] used in Immunoprecipitation (IP). GTX84657

Cat. No. GTX84657

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6D11

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, IP

Reactivity

 Human, Rat, Monkey
Package
100 μl ($519)
See all alpha B Crystallin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:50-1:100
IHC-P 1:50
FACS 1:100
IP 2ug/500ul
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human CRYAB (NP_001876) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

crystallin alpha B , CMD1II , CRYA2 , CTPP2 , CTRCT16 , HEL-S-101 , HSPB5 , MFM2

Cellular Localization

Cytoplasm,Nucleus

Background

May contribute to the transparency and refractive index of the lens.

Database

Research Area

DATA IMAGES

Anti-alpha B Crystallin antibody [6D11] used in IHC (Paraffin sections) (IHC-P). GTX84657

GTX84657 IHC-P Image

IHC-P analysis of human thyroid carcinoma tissue using GTX84657 alpha B Crystallin antibody [6D11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-alpha B Crystallin antibody [6D11] used in Flow cytometry (FACS). GTX84657

GTX84657 FACS Image

FACS analysis of HEK293T cells transfected with either alpha B Crystallin plasmid(Red) or empty vector control plasmid(Blue) using GTX84657 alpha B Crystallin antibody [6D11].

Anti-alpha B Crystallin antibody [6D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84657

GTX84657 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX84657 alpha B Crystallin antibody [6D11].

Anti-alpha B Crystallin antibody [6D11] used in IHC (Paraffin sections) (IHC-P). GTX84657

GTX84657 IHC-P Image

IHC-P analysis of human smooth muscle cancer tissue using GTX84657 alpha B Crystallin antibody [6D11].

Anti-alpha B Crystallin antibody [6D11] used in Western Blot (WB). GTX84657

GTX84657 WB Image

WB analysis of various cell lines using GTX84657 alpha B Crystallin antibody [6D11].
Loading : 35 ug per lane

Anti-alpha B Crystallin antibody [6D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84657

GTX84657 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with alpha B Crystallin plasmid using GTX84657 alpha B Crystallin antibody [6D11].

Anti-alpha B Crystallin antibody [6D11] used in Western Blot (WB). GTX84657

GTX84657 WB Image

Overexpression

WB analysis of HEK293T cells transfected with alpha B Crystallin plasmid (Right) or empty vector (Left) for 48 hrs using GTX84657 alpha B Crystallin antibody [6D11].
Loading : 5 ug per lane

Anti-alpha B Crystallin antibody [6D11] used in Western Blot (WB). GTX84657

GTX84657 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84657 alpha B Crystallin antibody [6D11].
Loading : 10 ug per lane
Dilution : 1:200

Anti-alpha B Crystallin antibody [6D11] used in Immunoprecipitation (IP). GTX84657

GTX84657 IP Image

IP analysis of DDDDK tagged alpha B Crystallin overexpressed HEK293T lysate using GTX84657 alpha B Crystallin antibody [6D11].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519