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alpha Glucosidase II antibody

Anti-alpha Glucosidase II antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66218
Anti-alpha Glucosidase II antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66218
Anti-alpha Glucosidase II antibody used in Western Blot (WB). GTX66218
Anti-alpha Glucosidase II antibody used in IHC (Paraffin sections) (IHC-P). GTX66218
Anti-alpha Glucosidase II antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66218
Anti-alpha Glucosidase II antibody used in IHC (Paraffin sections) (IHC-P). GTX66218

Cat. No. GTX66218

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all alpha Glucosidase II products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

107 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-260 of human GANAB (NP_001265123.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glucosidase II alpha subunit , G2AN , GIIA , GLUII , PKD3

Cellular Localization

Endoplasmic reticulum,Golgi apparatus

Background

This gene encodes the alpha subunit of glucosidase II and a member of the glycosyl hydrolase 31 family of proteins. The heterodimeric enzyme glucosidase II plays a role in protein folding and quality control by cleaving glucose residues from immature glycoproteins in the endoplasmic reticulum. Expression of the encoded protein is elevated in lung tumor tissue and in response to UV irradiation. Mutations in this gene cause autosomal-dominant polycystic kidney and liver disease. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-alpha Glucosidase II antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66218

GTX66218 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX66218 alpha Glucosidase II antibody.
Blue : DAPI
Dilution : 1:100

Anti-alpha Glucosidase II antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66218

GTX66218 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX66218 alpha Glucosidase II antibody.
Blue : DAPI
Dilution : 1:100

Anti-alpha Glucosidase II antibody used in Western Blot (WB). GTX66218

GTX66218 WB Image

WB analysis of various sample lysates using GTX66218 alpha Glucosidase II antibody.
Dilution : 1:3000
Loading : 25μg per lane

Anti-alpha Glucosidase II antibody used in IHC (Paraffin sections) (IHC-P). GTX66218

GTX66218 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX66218 alpha Glucosidase II antibody.
Dilution : 1:150

Anti-alpha Glucosidase II antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66218

GTX66218 ICC/IF Image

ICC/IF analysis of C6 cells using GTX66218 alpha Glucosidase II antibody.
Blue : DAPI
Dilution : 1:100

Anti-alpha Glucosidase II antibody used in IHC (Paraffin sections) (IHC-P). GTX66218

GTX66218 IHC-P Image

IHC-P analysis of rat brain tissue using GTX66218 alpha Glucosidase II antibody.
Dilution : 1:150

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399