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alpha Internexin antibody

Orthogonal
Anti-alpha Internexin antibody used in Western Blot (WB). GTX130053
Anti-alpha Internexin antibody used in Western Blot (WB). GTX130053
Anti-alpha Internexin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130053
Anti-alpha Internexin antibody used in Immunoprecipitation (IP). GTX130053
Anti-alpha Internexin antibody used in Western Blot (WB). GTX130053
Anti-alpha Internexin antibody used in Western Blot (WB). GTX130053
Anti-alpha Internexin antibody used in Western Blot (WB). GTX130053

Cat. No. GTX130053

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all alpha Internexin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:800
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , SK-N-AS , SH-SY5Y , mouse brain , rat brain

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human alpha Internexin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886165

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

internexin neuronal intermediate filament protein alpha , NEF5 , NF-66 , TXBP-1

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene is a member of the intermediate filament family and is involved in the morphogenesis of neurons. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-alpha Internexin antibody used in Western Blot (WB). GTX130053

GTX130053 WB Image

alpha Internexin antibody detects alpha Internexin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody (GTX130053) diluted by 1:1000.

Anti-alpha Internexin antibody used in Western Blot (WB). GTX130053

GTX130053 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody (GTX130053) diluted at 1:1000.

Anti-alpha Internexin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130053

GTX130053 ICC/IF Image

alpha Internexin antibody detects alpha Internexin protein expression by immunofluorescent analysis.
Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Red: alpha Internexin protein stained by alpha Internexin antibody (GTX130053) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-alpha Internexin antibody used in Immunoprecipitation (IP). GTX130053

GTX130053 IP Image

Immunoprecipitation of alpha Internexin protein from U87-MG whole cell extracts using 5 μg of alpha Internexin antibody (GTX130053).
Western blot analysis was performed using alpha Internexin antibody (GTX130053) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-alpha Internexin antibody used in Western Blot (WB). GTX130053

GTX130053 WB Image

alpha Internexin antibody detects alpha Internexin protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody (GTX130053) diluted by 1:1000.

Anti-alpha Internexin antibody used in Western Blot (WB). GTX130053

GTX130053 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody (GTX130053) diluted at 1:1000.

Anti-alpha Internexin antibody used in Western Blot (WB). GTX130053

GTX130053 WB Image

Orthogonal

The data was published in the journal Acta Neuropathol Commun in 2019. PMID: 30626447

REFERENCE

Application Reference

Gama Sosa MA et al. Acta Neuropathol Commun 2019; 7 (1) : 6 Low-level blast exposure disrupts gliovascular and neurovascular connections and induces a chronic vascular pathology in rat brain.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169