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alpha Synuclein antibody

Comparison
Anti-alpha Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX112799
Anti-alpha Synuclein antibody used in Western Blot (WB). GTX112799
Anti-alpha Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX112799
Anti-alpha Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX112799
Anti-alpha Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX112799
Anti-alpha Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX112799
Anti-alpha Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX112799
Anti-alpha Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX112799
Anti-alpha Synuclein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112799
Anti-alpha Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX112799
Anti-alpha Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX112799
Anti-alpha Synuclein antibody used in Western Blot (WB). GTX112799
Anti-alpha Synuclein antibody used in Western Blot (WB). GTX112799
Anti-alpha Synuclein antibody used in Western Blot (WB). GTX112799

Cat. No. GTX112799

Reference 10

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all alpha Synuclein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Sheep, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human alpha Synuclein Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618470

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synuclein alpha , NACP , PARK1 , PARK4 , PD1

Cellular Localization

Cytoplasm , Membrane , Nucleus

Background

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Four alternatively spliced transcripts encoding two different isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-alpha Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX112799

GTX112799 IHC-Fr Image

alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-alpha Synuclein antibody used in Western Blot (WB). GTX112799

GTX112799 WB Image

Comparison

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody (GTX112799) diluted at 1:2000 and competitor's antibody (MO22112) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-alpha Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX112799

GTX112799 IHC-P Image

alpha Synuclein antibody detects alpha Synuclein protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Green: alpha Synuclein antibody (GTX112799) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX112799

GTX112799 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using alpha Synuclein(GTX112799) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX112799

GTX112799 IHC-Fr Image

alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-alpha Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX112799

GTX112799 IHC-P Image

alpha Synuclein antibody detects alpha Synuclein protein at cytoplasm and nucleus in Rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded Rat brain.
alpha Synuclein antibody (GTX112799) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX112799

GTX112799 IHC-P Image

alpha Synuclein antibody detects alpha Synuclein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
alpha Synuclein stained by alpha Synuclein antibody (GTX112799) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX112799

GTX112799 IHC-Fr Image

alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
Anti-alpha Synuclein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112799

GTX112799 ICC/IF Image

alpha Synuclein antibody detects alpha Synuclein protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-alpha Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX112799

GTX112799 IHC-P Image

alpha Synuclein antibody detects alpha Synuclein protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
alpha Synuclein stained by alpha Synuclein antibody (GTX112799) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX112799

GTX112799 IHC-P Image

alpha Synuclein antibody detects alpha Synuclein protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
alpha Synuclein stained by alpha Synuclein antibody (GTX112799) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Synuclein antibody used in Western Blot (WB). GTX112799

GTX112799 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (GTX112799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Synuclein antibody used in Western Blot (WB). GTX112799

GTX112799 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (GTX112799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Synuclein antibody used in Western Blot (WB). GTX112799

GTX112799 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (GTX112799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

W Kim et al. Nat Commun 2023; 14 (1) : 4283 An optimized Nurr1 agonist provides disease-modifying effects in Parkinson’s disease models
Application : WB
Yin S et al. Mol Neurobiol 2022; (Epub) Cancerous Inhibitor of Protein Phosphatase 2A (CIP2A): Could It Be a Promising Biomarker and Therapeutic Target in Parkinson's Disease?
Application : WB
Reactivity : Human
Chen HY et al. J Biomed Sci 2021; 28 (1) : 65 Clinical and functional characterization of a novel STUB1 frameshift mutation in autosomal dominant spinocerebellar ataxia type 48 (SCA48).
Application : ICC/IF
Park SJ et al. PLoS Pathog 2021; 17 (10) : e1010018 Interaction between coxsackievirus B3 infection and α-synuclein in models of Parkinson's disease.
Reactivity : Mouse
Akiyama T et al. Stem Cells Transl Med 2021; 10 (4) : 572-581 Synthetic mRNA-based differentiation method enables early detection of Parkinson's phenotypes in neurons derived from Gaucher disease-induced pluripotent stem cells.
Application : WB
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REVIEW

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Application Reference
W Kim et al. Nat Commun 2023; 14 (1):4283 An optimized Nurr1 agonist provides disease-modifying effects in Parkinson’s disease models
Application : WB
Yin S et al. Mol Neurobiol 2022; (Epub) Cancerous Inhibitor of Protein Phosphatase 2A (CIP2A): Could It Be a Promising Biomarker and Therapeutic Target in Parkinson's Disease?
Application : WB
Reactivity : Human
Chen HY et al. J Biomed Sci 2021; 28 (1):65 Clinical and functional characterization of a novel STUB1 frameshift mutation in autosomal dominant spinocerebellar ataxia type 48 (SCA48).
Application : ICC/IF
Park SJ et al. PLoS Pathog 2021; 17 (10):e1010018 Interaction between coxsackievirus B3 infection and α-synuclein in models of Parkinson's disease.
Reactivity : Mouse
Akiyama T et al. Stem Cells Transl Med 2021; 10 (4):572-581 Synthetic mRNA-based differentiation method enables early detection of Parkinson's phenotypes in neurons derived from Gaucher disease-induced pluripotent stem cells.
Application : WB
Kwon Y et al. Cell Death Dis 2020; 11 (10):874 Amitriptyline interferes with autophagy-mediated clearance of protein aggregates via inhibiting autophagosome maturation in neuronal cells.
Application : ICC/IF
Reactivity : Human
Zeng H et al. BMC Genomic 2019; 201 (1):851 Transcriptomic analysis of α-synuclein knockdown after T3 spinal cord injury in rats.
Application : WB
Reactivity : Rat
Han C et al. J Mol Med (Berl) 2019; (Epub) Exosomes from patients with Parkinson's disease are pathological in mice.
Application : WB, IHC-P
Reactivity : Human
Lee S et al. Korean J Physiol Pharmacol 2017; 21 (6):625-632 MPTP-induced vulnerability of * neurons in A53T α-synuclein overexpressed mice with the potential involvement of DJ-1 downregulation.
Application : WB
Reactivity : Mouse
Bastias-Candia S et al. Environ Toxicol 2015; 30 (6):656-63 Combined exposure to agriculture pesticides, paraquat and maneb, induces alterations in the N/OFQ-NOPr and PDYN/KOPr systems in rats: Relevance to sporadic Parkinson's disease.
Application : WB
Reactivity : Rat
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169