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beta 3 Adrenergic Receptor antibody

Anti-beta 3 Adrenergic Receptor antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03468
Anti-beta 3 Adrenergic Receptor antibody used in Western Blot (WB). GTX03468
Anti-beta 3 Adrenergic Receptor antibody used in Flow cytometry (FACS). GTX03468
Anti-beta 3 Adrenergic Receptor antibody used in Flow cytometry (FACS). GTX03468

Cat. No. GTX03468

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($429)
See all beta 3 Adrenergic Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.25-0.5μg/ml
ICC/IF 2μg/ml
FACS 1-3μg/1x10⁶ cells
ELISA 0.1-0.5μg/ml
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄

Preservative

0.05mg sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human beta 3 Adrenergic Receptor/ADRB3 recombinant protein (Position: R179-S250).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adrenoceptor beta 3 , BETA3AR

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

The protein encoded by this gene belongs to the family of beta adrenergic receptors, which mediate catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor is located mainly in the adipose tissue and is involved in the regulation of lipolysis and thermogenesis. [provided by RefSeq, Feb 2009]

Database

Research Area

DATA IMAGES

Anti-beta 3 Adrenergic Receptor antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03468

GTX03468 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX03468 beta 3 Adrenergic Receptor antibody.
Antigen retireval : Enzyme antigen retrieval was performed using enzyme antigen retrieval reagent for 15 mins.
Blue : DAPI
Dilution : 2μg/mL

Anti-beta 3 Adrenergic Receptor antibody used in Western Blot (WB). GTX03468

GTX03468 WB Image

WB analysis of various sample lysates using GTX03468 beta 3 Adrenergic Receptor antibody.
Lane 1: HEK293 whole cell lysate
Lane 2 : rat liver tissue lysates
Lane 3 : mouse liver tissue lysate
Dilution : 0.5 μg/ml
Loading : 50μg per lane

Anti-beta 3 Adrenergic Receptor antibody used in Flow cytometry (FACS). GTX03468

GTX03468 FACS Image

FACS analysis of THP-1 cells using GTX03468 beta 3 Adrenergic Receptor antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled cell
Antibody amount : 1μg/1x10⁶ cells

Anti-beta 3 Adrenergic Receptor antibody used in Flow cytometry (FACS). GTX03468

GTX03468 FACS Image

FACS analysis of HeLa cells using GTX03468 beta 3 Adrenergic Receptor antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled cell
Antibody amount : 1μg/1x10⁶ cells

REFERENCE

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REVIEW

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Package List Price ($)
$ 429