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beta Actin antibody [RM112]

Anti-beta Actin antibody [RM112] used in Western Blot (WB). GTX33610
Anti-beta Actin antibody [RM112] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33610
Anti-beta Actin antibody [RM112] used in IHC (Paraffin sections) (IHC-P). GTX33610

Cat. No. GTX33610

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 RM112

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IP, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all beta Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:200
IHC-P 1:200
FACS Assay dependent
IP 1:200
ELISA Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A peptide corresponding to the N-terminus of β-actin

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2887726

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ACTB,BRWS1,PS1TP5BP1,actin beta,b Actin,β-Actin,F-actin,G-actin,Filamentous Actin,Gobular Actin,F actin

Cellular Localization

Cytoplasm,Cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-beta Actin antibody [RM112] used in Western Blot (WB). GTX33610

GTX33610 WB Image

WB analysis of A431 cell lysates using GTX33610 beta Actin antibody [RM112].
Dilution : 1:1000

Anti-beta Actin antibody [RM112] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33610

GTX33610 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33610 beta Actin antibody [RM112].
Red : Primary antibody
Blue : DAPI
Dilution : 1:200

Anti-beta Actin antibody [RM112] used in IHC (Paraffin sections) (IHC-P). GTX33610

GTX33610 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX33610 beta Actin antibody [RM112].
Dilution : 1:1000

REFERENCE

Application Reference

Cheng YJ et al. Oncotarget 2014; 5 (22) : 11564-75 high id1 expression, a generally negative prognostic factor, paradoxically predicts a favorable prognosis for adjuvant * plus * therapy in surgically treated lung cancer patients.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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Package List Price ($)
$ 399