*Optimal dilutions/concentrations should be determined by the researcher.
Application
Recommended Dilution
WB
1:1,000
ICC/IF
1:1,000
IHC-Fr
Assay dependent
Not tested in other applications.
Positive Control
MDBK
Product Note
The antibody does not cross-react with plakoglobin (γ-catenin).
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Chicken
Immunogen
recombinant chicken β-catenin.
Purification
Unpurified
Conjugation
Unconjugated
RRID
AB_385197
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
catenin beta 1 , CHBCAT
Background
The distinct peripheral cytosolic proteins, alpha, beta and gamma-catenin (102, 94 and 86 kD) found in many tissues bind to the conserved cytoplasmic tail domain of the cell-adhesion cadherins. Catenins link E-cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt-1 protooncogene. They are good candidates for mediating transduction of cell-cell contact positional signals to the cell interior. Absence of alpha-catenin is found in certain tumor cell lines and reduced levels in certain human carcinomas. Beta-catenin binds directly to the cytoplasmic tail of E-cadherin. It binds to the N-terminus of alpha-catenin and interacts with the protein product of the tumor suppressor gene APC. This interaction involves a 15-aa repeat in the APC. Beta-catenin cell levels seem to be controlled by APC. The central core region of beta-catenin is involved in mediation of cadherin-catenin complex interaction with EGFR.
![Anti-beta Catenin antibody [6F9] used in Western Blot (WB). GTX26300](/upload/website/prouct_img/small/GTX26300/GTX26300_20170605_WB_w_23060722_555.webp "Anti-beta Catenin antibody [6F9] used in Western Blot (WB). GTX26300")
![Anti-beta Catenin antibody [6F9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26300](/upload/website/prouct_img/small/GTX26300/GTX26300_20170605_ICCIF_w_23060722_958.webp "Anti-beta Catenin antibody [6F9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26300")
![Anti-beta Catenin antibody [6F9] used in Western Blot (WB). GTX26300](/upload/website/prouct_img/normal/GTX26300/GTX26300_20170605_WB_w_23060722_555.webp "Anti-beta Catenin antibody [6F9] used in Western Blot (WB). GTX26300")
![Anti-beta Catenin antibody [6F9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26300](/upload/website/prouct_img/normal/GTX26300/GTX26300_20170605_ICCIF_w_23060722_958.webp "Anti-beta Catenin antibody [6F9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26300")