APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3 μg/ml |
| 10 μg/ml |
| 1 μg/ml |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
120 kDa MBP/beta catenin fusion protein purified on amylose column.
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
catenin beta 1 , CTNNB , EVR7 , MRD19 , armadillo
Cellular Localization
Cytoplasm,Nucleus,Cytoplasm, cytoskeleton,Cell junction,Cell membrane,Cell junction, synapse
Background
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]
Database
Research Area
DATA IMAGES
![Anti-beta Catenin antibody [9F2] used in Western Blot (WB). GTX80686](/templates/default/js/lightbox/images/loading.gif "Anti-beta Catenin antibody [9F2] used in Western Blot (WB). GTX80686")
|
GTX80686 WB Image
WB analysis of whole cell extracts (30 μg lysate) of HeLa (Lane 1), U-87 MG (Lane 2), COLO 205 (Lane 3), HCT 116 (lane 4), HT-29 (lane 5), MCF7 (lane 6), MDA-MB-231 (lane 7) and A549 (lane 8) using GTX80686 beta Catenin antibody [9F2].
Dilution : 1-3 μg/ml
|
|
|
GTX80686 ICC/IF Image
ICC/IF analysis of U251 cells using GTX80686 beta Catenin antibody [9F2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC
|
|
|
GTX80686 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX80686 beta Catenin antibody [9F2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC
|
REFERENCE
There are currently no references for beta Catenin antibody [9F2] (GTX80686). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for beta Catenin antibody [9F2] (GTX80686). Be the first to share your experience with this product.
![Anti-beta Catenin antibody [9F2] used in Western Blot (WB). GTX80686](/upload/website/prouct_img/small/GTX80686/GTX80686_1951_WB_w_23061322_182.webp "Anti-beta Catenin antibody [9F2] used in Western Blot (WB). GTX80686")
![Anti-beta Catenin antibody [9F2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80686](/upload/website/prouct_img/small/GTX80686/GTX80686_896_ICC-IF_w_23061322_768.webp "Anti-beta Catenin antibody [9F2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80686")
![Anti-beta Catenin antibody [9F2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80686](/upload/website/prouct_img/small/GTX80686/GTX80686_895_ICC-IF_w_23061322_612.webp "Anti-beta Catenin antibody [9F2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80686")
![Anti-beta Catenin antibody [9F2] used in Western Blot (WB). GTX80686](/upload/website/prouct_img/normal/GTX80686/GTX80686_1951_WB_w_23061322_182.webp "Anti-beta Catenin antibody [9F2] used in Western Blot (WB). GTX80686")
![Anti-beta Catenin antibody [9F2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80686](/upload/website/prouct_img/normal/GTX80686/GTX80686_896_ICC-IF_w_23061322_768.webp "Anti-beta Catenin antibody [9F2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80686")
![Anti-beta Catenin antibody [9F2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80686](/upload/website/prouct_img/normal/GTX80686/GTX80686_895_ICC-IF_w_23061322_612.webp "Anti-beta Catenin antibody [9F2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80686")