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beta Catenin antibody

Cat. No. GTX26302

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, Dot

Reactivity

 Human, Mouse, Rat, Zebrafish, Pig, Xenopus laevis, Fish
Package
100 μl ($399)
See all beta Catenin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:4,000
ICC/IF 1:2,000
IHC-P Assay dependent
IHC-Fr 1:2,000
IP Assay dependent
Dot Assay dependent

Note :

IHC-Fr
using a denatured-reduced preparation
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

bovine kidney, MDBK

Product Note

The antibody does not cross react with α-catenin or γ-catenin (plakoglobin).

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

synthetic peptide corresponding to the C-terminal region (amino acids 768-781) of human/mouse β-catenin, conjugated with gluteraldehyde to KLH.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_385199

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

catenin beta 1 , CTNNB , EVR7 , MRD19 , armadillo

Cellular Localization

Cytoplasm,Nucleus,Cytoplasm, cytoskeleton,Cell junction,Cell membrane,Cell junction, synapse

Background

The distinct peripheral cytosolic proteins, alpha, beta and gamma-catenin (102, 94 and 86 kD) found in many tissues (1,2,3) bind to the conserved cytoplasmic tail domain of the cell-adhesion cadherins. Catenins link E-cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt-1 proto-oncogene. (6,7) They are good candidates for mediating transduction of cell-cell contact positional signals to the cell interior. (4,5) Absence of alpha-catenin is found in certain tumor cell lines (8) and reduced levels in certain human carcinomas (9). Beta-catenin binds directly to the cytoplasmic tail of E-cadherin. It binds to the N-terminus of alpha-catenin and interacts with the protein product of the tumor suppressor gene APC. (10) This interaction involves a 15-aa repeat in the APC. (11) Beta-catenin cell levels seem to be controlled by APC. (12) The central core region of beta-catenin is involved in mediation of cadherin-catenin complex interaction with EGFR. (13)

Database

Research Area

REFERENCE

Application Reference

Bloomquist RF et al. Proc Natl Acad Sci U S A 2019; 116 (36) : 17858-17866 Developmental plasticity of epithelial stem cells in tooth and taste bud renewal.
Application : IHC-P
Reactivity : Fish
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399