APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 0.5-1μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues require heating tissue sections in 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
A synthetic peptide from the middle of beta-Catenin (CTNNB1) protein
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CTNNB , CTNNB1 , MRD19 , armadillo , catenin beta 1 , beta Catenin , Catenin-β , β Catenin
Cellular Localization
Cytoplasm,Nucleus,Cytoskeleton,Cell junction,Adherens junction,Cell membrane,Microtubule organizing center,Centrosome,Spindle pole,Synapse
Background
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]
Database
Research Area
DATA IMAGES
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GTX34438 IHC-P Image
IHC-P analysis of breast ductal carcinoma tissue using GTX34438 beta Catenin antibody.
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GTX34438 FACS Image
FACS analysis of PFA-fixed HeLa cells using GTX34438 beta Catenin antibody.
Blue : Primary antibody
Red : Isotype control
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GTX34438 WB Image
WB analysis of human brain (1) absence, or (2) presence of immunizing peptide, and (3) mouse brain lysates using GTX34438 beta Catenin antibody.
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GTX34438 IHC-P Image
IHC-P analysis of breast lobular carcinoma tissue using GTX34438 beta Catenin antibody.
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REFERENCE
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REVIEW
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