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beta Galactosidase antibody

Overexpression
Anti-beta Galactosidase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00718
Anti-beta Galactosidase antibody used in Western Blot (WB). GTX00718
Anti-beta Galactosidase antibody used in Immunoprecipitation (IP). GTX00718
Anti-beta Galactosidase antibody used in ELISA (ELISA). GTX00718

Cat. No. GTX00718

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, ELISA

Reactivity

 E. coli
Package
200 μg ($399)
See all beta Galactosidase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF 1:200-1:500
IP 1:200-1:500
ELISA 1:2000-1:3000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full-size E. coli beta-Galactosidase

Purification

Protein A purified
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-beta Galactosidase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00718

GTX00718 ICC/IF Image

Overexpression

ICC/IF analysis of HEK293A cells transfected with beta-Gal cDNA using GTX00718 beta Galactosidase antibody.
Dilution : 1:500
Fixation : 4% PFA for 24 hrs
Permeabilization : Methanol

Anti-beta Galactosidase antibody used in Western Blot (WB). GTX00718

GTX00718 WB Image

WB analysis of various samples using GTX00718 beta Galactosidase antibody.
Lane 1 : Purified beta-galactosidase (10 ng)
Lane 2 : Uninduced E. coli K12 cell extract (30 μg)
Lane 3 : E.coli K12 cell extract induced by IPTG for beta-galactosidase expression (30 μg)
Dilution : 1:1000

Anti-beta Galactosidase antibody used in Immunoprecipitation (IP). GTX00718

GTX00718 IP Image

IP analysis of E. coli K12 cells induced beta-galactosidase expression by IPTG using GTX00718 beta Galactosidase antibody.
Lane 1 : Crude cell extract
Lane 2 : Supernatant of the immuno-precipitated E. coli crude extract
Lane 3 : Immuno-precipitate of crude E. coli extract
IP antibody : 1:500
IB antibody : 1:1000

Anti-beta Galactosidase antibody used in ELISA (ELISA). GTX00718

GTX00718 ELISA Image

ELISA analysis of beta-galactosidase using GTX00718 beta Galactosidase antibody.
Dilution : 100 μl
Coating : 100 ng

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399