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beta Lactoglobulin antibody

Anti-beta Lactoglobulin antibody used in ELISA (ELISA). GTX77272
Anti-beta Lactoglobulin antibody used in Western Blot (WB). GTX77272

Cat. No. GTX77272

Reference 11

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA, Functional Assay, Immunoelectrophoresis

Reactivity

 Bovine
Package
1 ml ($179)
See all beta Lactoglobulin products
PRODUCT

Summary

beta-Lactoglobulin antibody was generated against the bovine beta-lactoglobulin protein, the primary whey component in ruminant milk. beta-Lactoglobulin is typically used as an internal reference standard for quantification of human plasma protein in enzyme-linked immunosorbent assays (ELISAs) and mass spectrometric immunoassays.

APPLICATION

Application Note

Suggested starting dilutions are as follows: WB: 1:5000-1:20000, ELISA. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Positive Control

non-fat milk

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Beta lactoglobulin extract from bovine milk.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_373440

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

LGB antibody , MGC134239 antibody

Background

Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.

Research Area

DATA IMAGES

Anti-beta Lactoglobulin antibody used in ELISA (ELISA). GTX77272

GTX77272 ELISA Image

ELISA detection of Beta-lactoglobulin using GTX77272 for detection at a concentration of 0.2 μg/mL.

Anti-beta Lactoglobulin antibody used in Western Blot (WB). GTX77272

GTX77272 WB Image

Beta lactoglobulin antibody detects Beta lactoglobulin protein by Western blot analysis.Non-fat milk (0.5μg and 0.1μg and 0.05μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Beta lactoglobulin antibody (GTX77272) at a dilution of 1:10000.

REFERENCE

Application Reference

Morschheuser Lena et al. Journal of Chromatography A 2017; : Epub ahead of print Immunological analysis of food proteins using High-performance thin-layer chromatography-immunostaining.
Trenchevska O et al. Clin Mass Spectrom 2016; 1 : 27-31 Association of Cystatin C Proteoforms with Estimated Glomerular Filtration Rate.
Application : ELISA
Yassine HN et al. Proteome Sci 2016; 14 : 7 The association of plasma cystatin C proteoforms with diabetic chronic kidney disease.
Trenchevska O et al. Biomarkers 2016; (Epub) Development of quantitative mass spectrometric immunoassay for serum amyloid A.
Trenchevska O et al. PLoS One 2014; 9 (6) : e100713 Delineation of concentration ranges and longitudinal changes of human plasma protein variants.
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Application Reference
Morschheuser Lena et al. Journal of Chromatography A 2017;:Epub ahead of print Immunological analysis of food proteins using High-performance thin-layer chromatography-immunostaining.
Trenchevska O et al. Clin Mass Spectrom 2016; 1:27-31 Association of Cystatin C Proteoforms with Estimated Glomerular Filtration Rate.
Application : ELISA
Yassine HN et al. Proteome Sci 2016; 14:7 The association of plasma cystatin C proteoforms with diabetic chronic kidney disease.
Trenchevska O et al. Biomarkers 2016; (Epub) Development of quantitative mass spectrometric immunoassay for serum amyloid A.
Trenchevska O et al. PLoS One 2014; 9 (6):e100713 Delineation of concentration ranges and longitudinal changes of human plasma protein variants.
Gasilova N et al. Electrophoresis 2012; 33 (15):179183 Analysis of major milk whey proteins by immunoaffinity capillary electrophoresis coupled with MALDI-MS.
Yang Q et al. Endocrinology 2012; 153 (3):1519-27 quantitative measurement of full-length and c-terminal proteolyzed rbp4 in serum of normal and *-resistant humans using a novel mass spectrometry immunoassay.
Trenchevska O et al. Proteomics 2011; 11 (18):3633-41 Mass spectrometric immunoassay for quantitative determination of transthyretin and its variants.
Kiernan UA et al. PLoS One 2011; 6 (3):e17282 Quantitative mass spectrometry evaluation of human retinol binding protein 4 and related variants.
Trenchevska O et al. Proteome Sci 2011; 9 (1):19 Targeted quantitative mass spectrometric immunoassay for human protein variants.
Application : Functional Assay
Trenchevska O et al. J Proteome Res 2010; 9 (11):5969-73 Mass spectrometric immunoassay for quantitative determination of protein biomarker isoforms.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 179