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c-Abl antibody

Anti-c-Abl antibody used in IHC (Paraffin sections) (IHC-P). GTX111317
Anti-c-Abl antibody used in Western Blot (WB). GTX111317
Anti-c-Abl antibody used in Western Blot (WB). GTX111317
Anti-c-Abl antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111317

Cat. No. GTX111317

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all c-Abl products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

123 kDa. ( Note )

Positive Control

NT2D1 , K562

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human c-Abl. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722179

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ABL proto-oncogene 1, non-receptor tyrosine kinase , ABL , CHDSKM , JTK7 , bcr/abl , c-ABL , c-ABL1 , p150 , v-abl

Cellular Localization

Cytoplasm , cytoskeleton , Nucleus

Background

The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-c-Abl antibody used in IHC (Paraffin sections) (IHC-P). GTX111317

GTX111317 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using c-Abl(GTX111317) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-c-Abl antibody used in Western Blot (WB). GTX111317

GTX111317 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with c-Abl antibody (GTX111317) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-c-Abl antibody used in Western Blot (WB). GTX111317

GTX111317 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Abl antibody (GTX111317) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-c-Abl antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111317

GTX111317 ICC/IF Image

c-Abl antibody detects c-Abl protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: c-Abl protein stained by c-Abl antibody (GTX111317) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Hung AC et al. Clin Cancer Res 2016; (Epub) Extracellular Visfatin-Promoted Malignant Behavior in Breast Cancer Is Mediated Through c-Abl and STAT3 Activation.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169