c-Fos antibody

Cat. No. GTX129846

Reference 15

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-Fr
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all c-Fos products

View product citations for antibody GTX129846 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-Fr	1:100-1:1000

Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

MCF-7(500nM PMA treatment for 1hr)

Predict Reactivity

Sheep, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human c-Fos. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886108

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fos proto-oncogene, AP-1 transcription factor subunit , AP-1 , C-FOS , p55

Cellular Localization

Nucleus

Background

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq]

Database

Gene ID: 2353 FOS
UniProt: P01100 FOS

Research Area

DATA IMAGES

GTX129846 IHC-Fr Image

c-Fos antibody detects c-Fos protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebral cortex.
Green: c-Fos stained by c-Fos antibody (GTX129846) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX129846 ICC/IF Image

c-Fos antibody detects c-Fos protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: c-Fos stained by c-Fos antibody (GTX129846) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX129846 WB Image

Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with c-Fos antibody (GTX129846) diluted at 1:1000.

GTX129846 ICC/IF Image

c-Fos antibody detects c-Fos protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: c-Fos protein stained by c-Fos antibody (GTX129846) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.