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c-Jun (phospho Ser63) antibody [GT1198]

Recombinant antibody Orthogonal
Anti-c-Jun (phospho Ser63) antibody [GT1198] used in Western Blot (WB). GTX00960
Anti-c-Jun (phospho Ser63) antibody [GT1198] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00960
Anti-c-Jun (phospho Ser63) antibody [GT1198] used in Western Blot (WB). GTX00960

Cat. No. GTX00960

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1198

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all c-Jun products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Phospho-c-Jun (S63).

Purification


Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Jun proto-oncogene, AP-1 transcription factor subunit , AP1 , JUN , Jun protooncogene, AP1 transcription factor subunit , cJun , cjun

Cellular Localization

Nucleus

Background

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-c-Jun (phospho Ser63) antibody [GT1198] used in Western Blot (WB). GTX00960

GTX00960 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Jun (phospho Ser63) antibody [GT1198] (GTX00960) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-c-Jun (phospho Ser63) antibody [GT1198] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00960

GTX00960 ICC/IF Image

ICC/IF analysis of PFA-fixed C6 cells using GTX00960 c-Jun (phospho Ser63) antibody [GT1198].
Orange : Primary antibody
Blue : DAPI

Anti-c-Jun (phospho Ser63) antibody [GT1198] used in Western Blot (WB). GTX00960

GTX00960 WB Image

Orthogonal

WB analysis of NIH-3T3 cell extracts with/without Anisomycin (25 μg/ml, 37ºC, 30 min) treatment using GTX00960 c-Jun (phospho Ser63) antibody [GT1198].
Dilution : 1:1000
Loading : 25μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399