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delta 2 Catenin antibody [HL1629]

Recombinant antibody
Anti-delta 2 Catenin antibody [HL1629] used in Western Blot (WB). GTX637122
Anti-delta 2 Catenin antibody [HL1629] used in Western Blot (WB). GTX637122
Anti-delta 2 Catenin antibody [HL1629] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637122
Anti-delta 2 Catenin antibody [HL1629] used in Western Blot (WB). GTX637122

Cat. No. GTX637122

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1629

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all delta 2 Catenin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

133 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human delta 2 Catenin. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

catenin delta 2 , GT24 , NPRAP

Cellular Localization

Nucleus,Cell projection, dendrite

Background

This gene encodes an adhesive junction associated protein of the armadillo/beta-catenin superfamily and is implicated in brain and eye development and cancer formation. The protein encoded by this gene promotes the disruption of E-cadherin based adherens junction to favor cell spreading upon stimulation by hepatocyte growth factor. This gene is overexpressed in prostate adenocarcinomas and is associated with decreased expression of tumor suppressor E-cadherin in this tissue. This gene resides in a region of the short arm of chromosome 5 that is deleted in Cri du Chat syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013]

Database

Research Area

DATA IMAGES

Anti-delta 2 Catenin antibody [HL1629] used in Western Blot (WB). GTX637122

GTX637122 WB Image

Mouse tissue extract (50 Âμg) was separated by 5% SDS-PAGE, and the membrane was blotted with delta 2 Catenin antibody [HL1629] (GTX637122) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-delta 2 Catenin antibody [HL1629] used in Western Blot (WB). GTX637122

GTX637122 WB Image

Human tissue extract (30 Âμg) was separated by 5% SDS-PAGE, and the membrane was blotted with delta 2 Catenin antibody [HL1629] (GTX637122) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-delta 2 Catenin antibody [HL1629] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637122

GTX637122 ICC/IF Image

Delta 2 Catenin antibody [HL1629] detects delta 2 Catenin protein at dendrite by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: delta 2 Catenin stained by delta 2 Catenin antibody [HL1629] (GTX637122) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-delta 2 Catenin antibody [HL1629] used in Western Blot (WB). GTX637122

GTX637122 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with delta 2 Catenin antibody [HL1629] (GTX637122) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations