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eIF1 antibody [2B9]

Anti-eIF1 antibody [2B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54748
Anti-eIF1 antibody [2B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54748
Anti-eIF1 antibody [2B9] used in Western Blot (WB). GTX54748
Anti-eIF1 antibody [2B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54748
Anti-eIF1 antibody [2B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54748
Anti-eIF1 antibody [2B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54748
Anti-eIF1 antibody [2B9] used in Western Blot (WB). GTX54748

Cat. No. GTX54748

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2B9

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all eIF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1-2 μg/ml
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 30% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full lengthhuman EIF1 protein produced in HEK293T cells.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_2887881

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 1 , A121 , EIF-1 , EIF1A , ISO1 , SUI1

Database

DATA IMAGES

Anti-eIF1 antibody [2B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54748

GTX54748 ICC/IF Image

ICC/IF analysis of HeLa cells and NIH3T3 cells using GTX54748 eIF1 antibody [2B9].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

Anti-eIF1 antibody [2B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54748

GTX54748 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX54748 eIF1 antibody [2B9]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC

Anti-eIF1 antibody [2B9] used in Western Blot (WB). GTX54748

GTX54748 WB Image

WB analysis of 80μg whole cell lysate using GTX54748 eIF1 antibody [2B9].
Dilution : 1:1000

Anti-eIF1 antibody [2B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54748

GTX54748 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX54748 eIF1 antibody [2B9]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC

Anti-eIF1 antibody [2B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54748

GTX54748 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX54748 eIF1 antibody [2B9]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC

Anti-eIF1 antibody [2B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54748

GTX54748 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX54748 eIF1 antibody [2B9]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton„ X-100 for 10 minutes
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

Anti-eIF1 antibody [2B9] used in Western Blot (WB). GTX54748

GTX54748 WB Image

WB analysis of whole cell extracts (30 μg lysate) of A549 (Lane 1), Hep G2 (lane 2) and PANC-1 (Lane 3) using GTX54748 eIF1 antibody [2B9].
Dilution : 1:500-1:2000

REFERENCE

Application Reference

Pisareva VP et al. Nucleic Acids Res 2016; 44 (9) : 4252-65 DHX29 reduces leaky scanning through an upstream AUG codon regardless of its nucleotide context.
Application : WB
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399