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eIF2B3 antibody [1H3]

Anti-eIF2B3 antibody [1H3] used in IHC (Paraffin sections) (IHC-P). GTX54741
Anti-eIF2B3 antibody [1H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54741
Anti-eIF2B3 antibody [1H3] used in Western Blot (WB). GTX54741
Anti-eIF2B3 antibody [1H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54741
Anti-eIF2B3 antibody [1H3] used in Immunoprecipitation (IP). GTX54741
Anti-eIF2B3 antibody [1H3] used in IHC (Paraffin sections) (IHC-P). GTX54741

Cat. No. GTX54741

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1H3

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Rat, Primate
Package
100 μg ($399)
See all eIF2B3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:10-1:100
IHC-P 1:100-1:200
IP 3 μg
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 30% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human eIF2B3 produced in HEK293T cells.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 2B subunit gamma , EIF-2B , EIF2Bgamma

Background

The protein encoded by this gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-eIF2B3 antibody [1H3] used in IHC (Paraffin sections) (IHC-P). GTX54741

GTX54741 IHC-P Image

IHC-P analysis of human uterus tissue using GTX54741 eIF2B3 antibody [1H3].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-eIF2B3 antibody [1H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54741

GTX54741 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX54741 eIF2B3 antibody [1H3]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

Anti-eIF2B3 antibody [1H3] used in Western Blot (WB). GTX54741

GTX54741 WB Image

WB analysis of 80μg whole cell lysate using GTX54741 eIF2B3 antibody [1H3].
Dilution : 1:1000

Anti-eIF2B3 antibody [1H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54741

GTX54741 ICC/IF Image

ICC/IF analysis of COS7 cells using GTX54741 eIF2B3 antibody [1H3]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

Anti-eIF2B3 antibody [1H3] used in Immunoprecipitation (IP). GTX54741

GTX54741 IP Image

IP analysis of U2OS cell lysates using GTX54741 eIF2B3 antibody [1H3].
IP reaction : 3μg antibody / 500μg lysate
Dilution : 1:1000

Anti-eIF2B3 antibody [1H3] used in IHC (Paraffin sections) (IHC-P). GTX54741

GTX54741 IHC-P Image

IHC-P analysis of human breast tissue using GTX54741 eIF2B3 antibody [1H3].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399