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eIF3e antibody

Anti-eIF3e antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112342
Anti-eIF3e antibody used in IHC (Paraffin sections) (IHC-P). GTX112342
Anti-eIF3e antibody used in IHC (Paraffin sections) (IHC-P). GTX112342
Anti-eIF3e antibody used in Western Blot (WB). GTX112342
Anti-eIF3e antibody used in Western Blot (WB). GTX112342

Cat. No. GTX112342

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all eIF3e products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

Jurkat , Raji , BCL-1

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human eIF3e. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167212

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 3 subunit E , EIF3-P48 , EIF3S6 , INT6 , eIF3-p46

Cellular Localization

Cytoplasm , Nucleus

Background

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.

Database

Research Area

DATA IMAGES

Anti-eIF3e antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112342

GTX112342 ICC/IF Image

eIF3e antibody detects EIF3E protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: EIF3E protein stained by eIF3e antibody (GTX112342) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-eIF3e antibody used in IHC (Paraffin sections) (IHC-P). GTX112342

GTX112342 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using eIF3e(GTX112342) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-eIF3e antibody used in IHC (Paraffin sections) (IHC-P). GTX112342

GTX112342 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using eIF3e(GTX112342) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-eIF3e antibody used in Western Blot (WB). GTX112342

GTX112342 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
10% SDS PAGE
GTX112342 diluted at 1:2000

Anti-eIF3e antibody used in Western Blot (WB). GTX112342

GTX112342 WB Image

Sample (30 ug of whole cell lysate)
A: BCL-1
10% SDS PAGE
GTX112342 diluted at 1:2000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169