APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T cytoplasm fraction extract , mouse colon
Predict Reactivity
Rat, Rabbit, Bovine, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.09 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human eIF4E. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886563
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
eukaryotic translation initiation factor 4E , AUTS19 , CBP , EIF4E1 , EIF4EL1 , EIF4F , eIF-4E
Background
All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
|
GTX132092 ICC/IF Image
eIF4E antibody detects eIF4E protein at cytoplasm by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: eIF4E protein stained by eIF4E antibody (GTX132092) diluted at 1:500.
Blue: Hoechst 33342 staining.
|
|
|
GTX132092 IHC-P Image
eIF4E antibody detects EIF4E protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
eIF4E antibody (GTX132092) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX132092 WB Image
293T whole cell and cytoplasm extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody (GTX132092) diluted at 1:1000.
|
|
|
GTX132092 WB Image
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody (GTX132092) diluted at 1:1000.
|
REFERENCE
REVIEW
There are currently no reviews for eIF4E antibody (GTX132092). Be the first to share your experience with this product.
