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eNOS (phospho Ser1177) antibody

Orthogonal
Anti-eNOS (phospho Ser1177) antibody used in Western Blot (WB). GTX50212
Anti-eNOS (phospho Ser1177) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50212

Cat. No. GTX50212

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all eNOS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

133 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 1177 (T-Q-S(p)-F-S) derived from human eNOS.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nitric oxide synthase 3 , ECNOS , eNOS

Cellular Localization

Cell membrane,Cytoplasm, cytoskeleton,Golgi apparatus

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]

Database

Research Area

DATA IMAGES

Anti-eNOS (phospho Ser1177) antibody used in Western Blot (WB). GTX50212

GTX50212 WB Image

WB analysis of extracts from 3T3 cells untreated and treated with EGF HUVEC cells treated with VEGF JK cells untreated and treated with serum using GTX50212 eNOS (phospho Ser1177) antibody.

Anti-eNOS (phospho Ser1177) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50212

GTX50212 ICC/IF Image

Orthogonal

The data was published in the journal J Am Heart Assoc in 2019. PMID: 30885039

REFERENCE

Application Reference

Nobuyuki Masaki et al. J Am Heart Assoc. 2019; 8 (6) Endothelial Insulin Resistance of Freshly Isolated Arterial Endothelial Cells From Radial Sheaths in Patients With Suspected Coronary Artery Disease
Application : ICC/IF
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399