GeneTex

  • Country / Location Selection

United States (US)

gamma 2 Actin antibody

Anti-gamma 2 Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55849
Anti-gamma 2 Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX55849
Anti-gamma 2 Actin antibody used in Western Blot (WB). GTX55849

Cat. No. GTX55849

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all gamma 2 Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of gamma 2 Actin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin gamma 2, smooth muscle , ACT , ACTA3 , ACTE , ACTL3 , ACTSG , VSCM

Cellular Localization

Cytoplasm, cytoskeleton

Background

Actins are highly conserved proteins that are involved in various types of cell motility and in the maintenance of the cytoskeleton. Three types of actins, alpha, beta and gamma, have been identified in vertebrates. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. This gene encodes actin gamma 2; a smooth muscle actin found in enteric tissues. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Based on similarity to peptide cleavage of related actins, the mature protein of this gene is formed by removal of two N-terminal peptides.[provided by RefSeq, Dec 2010]

Database

  • Gene ID: 72 ACTG2
  • UniProt: P63267 ACTG2

Research Area

DATA IMAGES

Anti-gamma 2 Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55849

GTX55849 ICC/IF Image

ICC/IF analysis of formalin-fixed HeLa cells using GTX55849 gamma 2 Actin antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-gamma 2 Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX55849

GTX55849 IHC-P Image

IHC-P analysis of formalin fixed human muscle tissue section using GTX55849 gamma 2 Actin antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-gamma 2 Actin antibody used in Western Blot (WB). GTX55849

GTX55849 WB Image

WB analysis of DLD (A), HeLa (B), mouse brain (C), rat brain (D) whole cell lysates using GTX55849 gamma 2 Actin antibody.

REFERENCE

Application Reference

Wen Li et al. J Muscle Res Cell Motil 2021; 42 (2) : 219-231 Mfge8 attenuates human gastric antrum smooth muscle contractions.
Application : WB, IHC-Fr
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for gamma 2 Actin antibody (GTX55849). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399