GeneTex

  • Country / Location Selection

United States (US)

gamma Tubulin antibody [4D11]

KO/KD
Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794
Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794
Anti-gamma Tubulin antibody [4D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15794
Anti-gamma Tubulin antibody [4D11] used in IHC (Paraffin sections) (IHC-P). GTX15794
Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794
Anti-gamma Tubulin antibody [4D11] used in IHC (Paraffin sections) (IHC-P). GTX15794

Cat. No. GTX15794

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4D11

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IP, IHC

Reactivity

 Human, Mouse, Rat, Primate
Package
100 μg ($399)
See all gamma Tubulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:50
IHC-P 1:200
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein containing residues A A T R P D Y I S W G T Q E Q of human gamma Tubulin.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin gamma 1 , CDCBM4 , GCP-1 , TUBG , TUBGCP1

Background

This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq, Jan 2009]

Database

Research Area

DATA IMAGES

Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794

GTX15794 WB Image

WB analysis of 50 ug of various whole cell lysates using GTX15794 gamma Tubulin antibody [4D11].
Dilution : 1:1000

Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794

GTX15794 WB Image

WB analysis of membrane enriched extracts (30 ug lysate) of A-549 (Lane 1), MCF-7 (Lane 2), HeLa (Lane 3), HepG2 (Lane 4), Caco2 (Lane 5), A-431 (Lane 6), SH-SY5Y (Lane 7) and U-2 OS (Lane 8) using GTX15794 gamma Tubulin antibody [4D11].
Dilution : 1 μg/ml

Anti-gamma Tubulin antibody [4D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15794

GTX15794 ICC/IF Image

ICC/IF analysis of HeLa (A) and U2-OS cells (B) using GTX15794 gamma Tubulin antibody [4D11].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

Anti-gamma Tubulin antibody [4D11] used in IHC (Paraffin sections) (IHC-P). GTX15794

GTX15794 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX15794 gamma Tubulin antibody [4D11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794

GTX15794 WB Image

KO/KD

WB analysis of gamma tubulin knockdown HeLa cells (lane 3), scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1) using GTX15794 gamma Tubulin antibody [4D11].
Dilution : 1:1000

Anti-gamma Tubulin antibody [4D11] used in IHC (Paraffin sections) (IHC-P). GTX15794

GTX15794 IHC-P Image

IHC-P analysis of human colon tissue using GTX15794 gamma Tubulin antibody [4D11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

REFERENCE

There are currently no references for gamma Tubulin antibody [4D11] (GTX15794). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for gamma Tubulin antibody [4D11] (GTX15794). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399