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mAChR M2 antibody [B8E5]

Cat. No. GTX54482

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 B8E5

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-Fr, ELISA, Functional Assay

Reactivity

 Human, Mouse, Rat, Guinea pig
Package
50 μg ($399)
See all mAChR M2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
ELISA Assay dependent
Functional Assay Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Product Note

Monoclonal antibody B8E5 acts by functional dimerization of the receptor resulting in stabilization of the constitutive active receptor dimers and paradoxically induces a small decrease in carbachol affinity for the M2 receptor. It recognizes the pentapeptide VRTVE (aa 168-172) corresponding to the N-terminal part of the second extracellular loop of the human M2 receptor.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 0.1% BSA

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide VRTVEDGECYIQFFSNAAVTFGTAI(C): Human second extracellular loop residues 168-192

Purification

Purified IgG2a

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CHRM2,HM2,cholinergic receptor muscarinic 2,mAChR M2,Muscarinic Acetylcholine Receptor 2

Cellular Localization

Cell membrane,Cell junction,Synapse,Postsynaptic cell membrane

Background

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE

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REVIEW

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Package List Price ($)
$ 399