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mGluR3 antibody [C3], C-term

Anti-mGluR3 antibody [C3], C-term used in Western Blot (WB). GTX108212
Anti-mGluR3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108212
Anti-mGluR3 antibody [C3], C-term used in Western Blot (WB). GTX108212

Cat. No. GTX108212

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all mGluR3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

99 kDa. ( Note )

Positive Control

293T , A431 , mouse brain , rat brain

Predict Reactivity

Zebrafish, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human mGluR3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950457

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate metabotropic receptor 3 , GLUR3 , GPRC1C , MGLUR3 , mGlu3

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-mGluR3 antibody [C3], C-term used in Western Blot (WB). GTX108212

GTX108212 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:A431 (GTX27909)
7.5% SDS PAGE
GTX108212 diluted at 1:500

Anti-mGluR3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108212

GTX108212 ICC/IF Image

mGluR3 antibody [C3], C-term detects mGluR3 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: mGluR3 stained by mGluR3 antibody [C3], C-term (GTX108212) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-mGluR3 antibody [C3], C-term used in Western Blot (WB). GTX108212

GTX108212 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mGluR3 antibody [C3], C-term (GTX108212) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169