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nm23-H2 antibody [AT2E3]

Anti-nm23-H2 antibody [AT2E3] used in Western Blot (WB). GTX57712
Anti-nm23-H2 antibody [AT2E3] used in Western Blot (WB). GTX57712
Anti-nm23-H2 antibody [AT2E3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57712

Cat. No. GTX57712

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT2E3

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all nm23-H2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT2E3 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NME4 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NME/NM23 nucleoside diphosphate kinase 2 , NDKB , NDPKB , NM23B , NM23H2 , NME2 , PUF , nm23-H2

Cellular Localization

Cytoplasm,Nucleus,Cell projection,Lamellipodium,Ruffle

Background

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Nov 2010]

Database

Research Area

DATA IMAGES

Anti-nm23-H2 antibody [AT2E3] used in Western Blot (WB). GTX57712

GTX57712 WB Image

WB analysis of various samples using GTX57712 nm23-H2 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : A549 whole cell lysate
Lane 3 : MCF-7 whole cell lysate
Lane 4 : LNCap whole cell lysate
Loading : 40 μg
Dilution : 1:1000

Anti-nm23-H2 antibody [AT2E3] used in Western Blot (WB). GTX57712

GTX57712 WB Image

WB analysis of various samples using GTX57712 nm23-H2 antibody.
Lane 1 : 293T cell lysate
Lane 2 : NME2 transfected 293T cell lysate
Loading : 5 μg
Dilution : 1:1000

Anti-nm23-H2 antibody [AT2E3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57712

GTX57712 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57712 nm23-H2 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399