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p190-B RhoGAP antibody

Anti-p190-B RhoGAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65862
Anti-p190-B RhoGAP antibody used in Western Blot (WB). GTX65862
Anti-p190-B RhoGAP antibody used in IHC (Paraffin sections) (IHC-P). GTX65862

Cat. No. GTX65862

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all p190-B RhoGAP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

172 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1292-1501 of human ARHGAP5 (NP_001164.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rho GTPase activating protein 5 , GFI2 , RhoGAP5 , p190-B , p190BRhoGAP

Cellular Localization

Cytoplasm,Cell membrane

Background

Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

  • Gene ID: 394 ARHGAP5
  • UniProt: Q13017 ARHGAP5

Research Area

DATA IMAGES

Anti-p190-B RhoGAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65862

GTX65862 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX65862 p190-B RhoGAP antibody.
Blue : DAPI
Dilution : 1:100

Anti-p190-B RhoGAP antibody used in Western Blot (WB). GTX65862

GTX65862 WB Image

WB analysis of various sample lysates using GTX65862 p190-B RhoGAP antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-p190-B RhoGAP antibody used in IHC (Paraffin sections) (IHC-P). GTX65862

GTX65862 IHC-P Image

IHC-P analysis of rat brain tissue using GTX65862 p190-B RhoGAP antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399