APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:1000 |
| 1:100 - 1:500 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol
Preservative
0.01% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of p38 MAPK. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mitogen-activated protein kinase 14 , CSBP , CSBP1 , CSBP2 , CSPB1 , EXIP , Mxi2 , PRKM14 , PRKM15 , RK , SAPK2A , p38 , p38ALPHA
Cellular Localization
Cytoplasm,Nucleus
Background
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX32319 WB Image
WB analysis of PMA-treated HepG2 (A), NIH3T3 (B), PC12 (C) whole cell lysates using GTX32319 p38 MAPK (phospho Thr180) antibody.
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GTX32319 ICC/IF Image
ICC/IF analysis of formalin-fixed HepG2 cells using GTX32319 p38 MAPK (phospho Thr180) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
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REFERENCE
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REVIEW
There are currently no reviews for p38 MAPK (phospho Thr180) antibody (GTX32319). Be the first to share your experience with this product.
