APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:1000 |
| 1:50 - 1:100 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human p38 MAPK around the phosphorylation site of tyrosine 182 (T-G-Yp-V-A).
Purification
Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum
Conjugation
Unconjugated
RRID
AB_11169423
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CSBP,CSBP1,CSBP2,CSPB1,EXIP,MAPK14,Mxi2,PRKM14,PRKM15,RK,SAPK2A,mitogenactivated protein kinase 14,p38,p38ALPHA,p38 MAPK,p38 alpha,mitogen-activated protein kinase 14
Cellular Localization
Cytoplasm,Nucleus
Background
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX78989 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX78989 p38 MAPK (phospho Tyr182) antibody.
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GTX78989 WB Image
WB analysis of NIH-3T3 (Line 1, 4, 7 and 10), COS7 (Line 2, 5, 8 and 11), and K562 (Line 3, 6, 9 and 12) cells, untreated or treated with UV (20min) lysates using GTX78989 p38 MAPK (phospho Tyr182) antibody.
Lane 1 - 6 : p38 MAPK antibody
Lane 7 - 12 : GTX78989 p38 MAPK (phospho Tyr182) antibody
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REFERENCE
There are currently no references for p38 MAPK (phospho Tyr182) antibody (GTX78989). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for p38 MAPK (phospho Tyr182) antibody (GTX78989). Be the first to share your experience with this product.
