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p73 delta N antibody [38C674.2]

Anti-p73 delta N antibody [38C674.2] used in IHC (Paraffin sections) (IHC-P). GTX11997
Anti-p73 delta N antibody [38C674.2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11997
Anti-p73 delta N antibody [38C674.2] used in Western Blot (WB). GTX11997
Anti-p73 delta N antibody [38C674.2] used in IHC (Paraffin sections) (IHC-P). GTX11997
Anti-p73 delta N antibody [38C674.2] used in IHC (Frozen sections) (IHC-Fr). GTX11997

Cat. No. GTX11997

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 38C674.2

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
50 μg ($399)
See all p73 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This antibody was developed against a peptide corresponding to a portion of amino acid residues 1-50 of human dNp73. Mouse and human sequences are more than 95 % identical at these amino acid residues. In mouse, alanine at position 8 is replaced by methionine.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Even though the tumor suppressor gene p53 is highly important in human cancer, as indicated by the fact that it is mutated in about 50% of cases, up to a few years ago no similar proteins had been identified. Two p53 homologues are now identified, p73 and p63, with high amino acid identity suggesting similar functions. Both p63 and p73 have two significant structural peculiarities compared with p53; the presence of an extended non-conserved C-terminus containing a sterile alpha motive (SAM), (typical of developmental proteins), and the presence of number of different splicing isoforms differing in the N-terminus or in the absence of the transactivation domain (delta N forms).

Research Area

DATA IMAGES

Anti-p73 delta N antibody [38C674.2] used in IHC (Paraffin sections) (IHC-P). GTX11997

GTX11997 IHC-P Image

IHC-P analysis of human lung squamous cell carcinoma tissue using GTX11997 p73 delta N antibody [38C674.2].
Dilution : 5 μg/ml

Anti-p73 delta N antibody [38C674.2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11997

GTX11997 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX11997 p73 delta N antibody [38C674.2].
Green : primary antibody
Red : Actin
Blue : DAPI
Dilution : 1:50
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100

Anti-p73 delta N antibody [38C674.2] used in Western Blot (WB). GTX11997

GTX11997 WB Image

WB analysis of (A) p73 delta N overexpressing (B) HeLa, and (C) NIH-3T3 cell lysate using GTX11997 p73 delta N antibody [38C674.2].
Dilution : 1 μg/ml

Anti-p73 delta N antibody [38C674.2] used in IHC (Paraffin sections) (IHC-P). GTX11997

GTX11997 IHC-P Image

IHC-P analysis of human placenta tissue using GTX11997 p73 delta N antibody [38C674.2].
Dilution : 5 μg/ml
Antigen retrieval : Heat-induced antigen retrieval

Anti-p73 delta N antibody [38C674.2] used in IHC (Frozen sections) (IHC-Fr). GTX11997

GTX11997 IHC-Fr Image

IHC-Fr analysis of E15 (A) and E17 (B) rat embryonic kidney tissue using GTX11997 p73 delta N antibody [38C674.2]. Delta Np73 is expressed in ureteric bud (UB) branches, and metanephrogenic mesenchyme (MM), as well as in cells invading the glomerular cleft. DeltaNp73 is expressed in the ureteric bud (UB) branches, metanephrogenic mesenchyme (MM), and nephron progenitors.
Dilution : 1:2000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399