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pan Cytokeratin antibody [C-11]

Anti-pan Cytokeratin antibody [C-11] used in Western Blot (WB). GTX48825
Anti-pan Cytokeratin antibody [C-11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48825
Anti-pan Cytokeratin antibody [C-11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48825
Anti-pan Cytokeratin antibody [C-11] used in Western Blot (WB). GTX48825
Anti-pan Cytokeratin antibody [C-11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48825

Cat. No. GTX48825

Reference 16

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 C-11

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP, ELISA, Multiplexing

Reactivity

 Human
Package
50 μg ($309)
See all pan Cytokeratin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:50-1:200
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
IP 1:100
ELISA 1:5000-1:20000
Multiplexing Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Product Note

This antibody reacts with keratin (56 kDa), keratin 17 (46 kDa), keratin 18 (45 kDa) and keratin 19 (40 kDa) derived from humans.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human cytoskeletal preparations from A431 cells.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_11178438

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 6C , K6E , KRT6E , PPKNEFD

Background

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (i.e. skin) and non-keratinizing cells (i.e. mesothelial)

Database

Research Area

DATA IMAGES

Anti-pan Cytokeratin antibody [C-11] used in Western Blot (WB). GTX48825

GTX48825 WB Image

Western blot using GeneTex Immunochemical's Mouse Mab-anti-Keratin antibody. This antibody recognizes a single 56 kDa band corresponding to human keratin as confirmed by the position of molecular weight markers (not shown). Approximatley 100 μg of keratin from human epidermis was applied under reducing conditions. 1:400 dilution of Mab anti-Keratin was used for 2h followed by detection using a 1:5,000 dilution of IRDyeTM800 conjugated Goat anti-Mouse IgG [H&L] and visualization using the OdysseyR Infrared Imaging System developed by LI-COR.

Anti-pan Cytokeratin antibody [C-11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48825

GTX48825 ICC/IF Image

Immunofluorescence microscopy using GeneTex Immunochemical's Mouse Mab-anti-Keratin antibody. Confocal slices of HeLa cells are between 0.5 and 0.6 um where the image is taken near the bottom of the cell. Use FITC a 1:2,000 dilution of FITC-conjugated Goat anti-Mouse IgG [H&L] for detection.

Anti-pan Cytokeratin antibody [C-11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48825

GTX48825 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX48825 pan Cytokeratin antibody [C-11].

Anti-pan Cytokeratin antibody [C-11] used in Western Blot (WB). GTX48825

GTX48825 WB Image

WB analysis of recombinant human pan Cytokeratin protein using GTX48825 pan Cytokeratin antibody [C-11].
Loading : 100 ng
Dilution : 1:400

Anti-pan Cytokeratin antibody [C-11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48825

GTX48825 ICC/IF Image

Immunofluorescence Microscopy of GeneTex Anti-Keratin antibody (GTX48825) was used with GeneTex Dylight 488 goat anti-mouse (shown in green) to detect Keratin by Immunofluorescence. In the same experiment, GeneTex polyclonal Anti-HDAC-1 antibody was used with Anti-Rabbit IgG (shown in red) to detect HDAC-1.

REFERENCE

Application Reference

Yang KS et al. Sci Rep 2023; 13 (1) : 10969 Extracellular vesicle analysis of plasma allows differential diagnosis of atypical pancreatic serous cystadenoma.

Clone Reference

Michael E B FitzPatrick et al. Cell Rep 2021; 34 (3):108661 Human intestinal tissue-resident memory T?cells comprise transcriptionally and functionally distinct subsets.
Franken A et al. Cancers (Basel) 2021; 13 (23) Multiparametric Circulating Tumor Cell Analysis to Select Targeted Therapies for Breast Cancer Patients.
Ng WL et al. Int J Bioprint 2021; 7 (2):332 Fabrication and Characterization of 3D Bioprinted Triple-layered Human Alveolar Lung Models.
Campello E et al. Dig Liver Dis 2021; (Epub) Acute kidney injury is associated with increased levels of circulating microvesicles in patients with decompensated cirrhosis.
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REVIEW

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Application Reference
Yang KS et al. Sci Rep 2023; 13 (1):10969 Extracellular vesicle analysis of plasma allows differential diagnosis of atypical pancreatic serous cystadenoma.
Ordonez NG et al. Hum Pathol 2013; 44 (7):1195-215 Broad-spectrum immunohistochemical epithelial markers: a review.
Application : IHC-P
Clone Reference
Michael E B FitzPatrick et al. Cell Rep 2021; 34 (3):108661 Human intestinal tissue-resident memory T?cells comprise transcriptionally and functionally distinct subsets.
Franken A et al. Cancers (Basel) 2021; 13 (23) Multiparametric Circulating Tumor Cell Analysis to Select Targeted Therapies for Breast Cancer Patients.
Ng WL et al. Int J Bioprint 2021; 7 (2):332 Fabrication and Characterization of 3D Bioprinted Triple-layered Human Alveolar Lung Models.
Campello E et al. Dig Liver Dis 2021; (Epub) Acute kidney injury is associated with increased levels of circulating microvesicles in patients with decompensated cirrhosis.
Labib M et al. Nat Biomed Eng 2020; (Epub) Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting.
Franken A et al. J Mol Diagn 2019; (Epub) Detection of ESR1 Mutations in Single Circulating Tumor Cells on Estrogen Deprivation Therapy but Not in Primary Tumors from Metastatic Luminal Breast Cancer Patients.
Szczerba BM et al. Nature 2019; (Epub) Neutrophils escort circulating tumour cells to enable cell cycle progression.
Labib M et al. Nat Chem 2018; 10 (5):489-495 Single-cell mRNA cytometry via sequence-specific nanoparticle clustering and trapping.
Green BJ et al. ACS Appl Mater Interfaces 2017; 9 (24):20435-20443 Isolation of Phenotypically Distinct Cancer Cells Using Nanoparticle-Mediated Sorting.
Blassl C et al. Mol Oncol 2016; (Epub) Gene expression profiling of single circulating tumor cells in ovarian cancer - Establishment of a multi-marker gene panel.
Neumann MH et al. Biotechnol Prog 2016; (Epub) Isolation and characterization of circulating tumor cells using a novel workflow combining the CellSearchR system and the CellCelector?.
Chebouti I et al. Oncotarget 2016; (Epub) Analysis of disseminated tumor cells before and after platinum based chemotherapy in primary ovarian cancer. Do stem cell like cells predict prognosis?
Schneck H et al. PLoS One 2015; 10 (12):e0144535 EpCAM-Independent Enrichment of Circulating Tumor Cells in Metastatic Breast Cancer.
Besant JD et al. Nanoscale 2015; 7 (14):6278-85 Velocity valleys enable efficient capture and spatial sorting of nanoparticle-bound cancer cells.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 309